UPLC-MS法测定全蝎中β-肌动蛋白含量的不确定度评定  

Uncertainty Evaluation of the Determination of β-Actin in Scorpion by UPLC-MS

作　　者：梁瑞强[1] 罗娇依 李梦怡[1] 林瑞超 曹进[1] 孙姗姗[1] Liang Ruiqiang;Luo Jiaoyi;Li Mengyi;Lin Ruichao;Cao Jin;Sun Shanshan(Key Laboratory for Food Quality and Safety of State Market Regulation,National Institute for Food and Drug Control,Beijing 100050,China;Beijing Key Lab for Quality Evalua-tion of Chinese Materia Medica,School of Chinese Materia Medica,Beijing University of Chinese Medicine)

机构地区：[1]中国食品药品检定研究院,国家市场监管重点实验室(食品质量与安全),北京100050 [2]北京中医药大学中药学院中药品质评价北京市重点实验室

出　　处：《中国药师》2022年第12期2117-2122,2157,共7页China Pharmacist

基　　金：国家中药标准化项目(编号:ZYBZH-C-QIN-45)。

摘　　要：目的:对用超高效液相色谱串联质谱法(UPLC-MS)测定中药全蝎中β-肌动蛋白含量的方法进行不确定度评定。方法:用UPLC-MS法测定含量,建立数学模型并分析方法流程以确定不确定度的来源,计算合成不确定度,并得出扩展不确定度。结果:用该方法测定全蝎中β-肌动蛋白含量的扩展不确定度为6.99 mg·kg^(-1),k=2。结论:检测结果的不确定度来源主要为供试品溶液测定浓度、供试品前处理过程加液和方法重复性。Objective: To establish an uncertainty analysis method for the determination of β-actin in scorpion by ultra high performance liquid chromatography tandem mass spectrometry(UPLC-MS).Methods: The content was determined by UPLC-MS, a mathematical model was established, and the method process was analyzed to determine the sources of uncertainty, calculate the synthetic uncertainty and obtain the extended uncertainty. Results: The expanded uncertainty of β-actin content in scorpion determined by this method was 6.99 mg·kg^(-1), k=2. Conclusion: The main uncertainty sources of the test results are the measured concentration of the test sample solution, the addition of liquid in the pretreatment process of the test sample and the repeatability of the method.

关 键 词：超高效液相色谱串联质谱法 全蝎 Β-肌动蛋白 不确定度 

分 类 号：R927.2[医药卫生—药学]