PUMA PROMOTES BAX ACTIVATION IN A FOXO3a-DEPENDENT MANNER IN STS-INDUCED APOPTOSIS  

作　　者：YINGJIE ZHANG DA XING 

机构地区：[1]MOE Key Laboratory of Laser Life Science and Institute of Laser Life Science College of Biophotonics,South China Normal University Guangzhou 510631,China

出　　处：《Journal of Innovative Optical Health Sciences》2010年第1期31-38,共8页创新光学健康科学杂志（英文）

基　　金：supported by the National Basic Research Program of China(2010CB732602);the Program for Changjiang Scholars and Innovative Research Team in University(IRT0829);the National Natural Science Foundation of China(30870676;30870658).

摘　　要：PUMA(p53 up-regulated modulator of apoptosis,also called Bbc3)was first identified as a BH3-only Bcl-2 family protein that is transcriptionally up-regulated by p53 and activated upon p53-dependent apoptotic stimuli,such as treatment with DNA-damaging drugs or UV irradiation.Recently,studies have shown that PUMA is also up-regulated in response to certain p53-independent apoptotic stimuli,such as growth factor deprivation or treatment with glucocorticoids or STS(staurosporine).However,the molecular mechanisms of PUMA up-regulation and how PUMA functions in response to p53-independent apoptotic stimuli remain poorly understood.In this study,based on real-time single cell analysis,flow cytometry,and western blotting technique,we investigated the function of PUMA in living human lung adenocarcinoma cells(ASTC-a-1)after STS treatment.Our results show that FOXO3a was activated by STS stimulation and then translocated from cytosol to nucleus.The expression of PUMA was up-regulated via a FOXO3a-dependent manner after STS treatment,while p53 had little function in this process.Moreover,cell apoptosis and Bax activation induced by STS were not blocked by Pifithrin-α(p53 inhibitor),which indicated that p53 was not involved in this signaling pathway.Taken together,these results suggest that PUMA promoted Bax activation in a FOXO3a-dependent pathway during STS-induced apoptosis,while p53 was dispensable in this process.

关 键 词：PUMA Bax activation FOXO3A STS apoptosis 

分 类 号：R73[医药卫生—肿瘤]