An automated multiwell plate reading flim microscope for live cell autofluorescence lifetime assays  

作　　者：Douglas J.Kelly Sean C.Warren Sunil Kumar Joao L.Lagarto Benjamin T.Dyer Anca Margineanu Eric W.-F.Lam Chris Dunsby Paul M.W.French 

机构地区：[1]Photonics Group,Department of Physics,Imperial College London South Kensington Campus,London,SW72AZ,UK [2]Institute of Chemical Biology,Department of Chemistry Imperial College London,South Kensington Campus London,SWT 2AZ,UK [3]National Heart&Lung Institute Imperial Centre for Eaperimental&Translational Medicine Du Cane Road,London,W12 ONN,UK [4]Department of Surgery and Cancer,Imperial Cllege London Du Cane Road,London,W12 ONN,UK [5]Centre for Histopathology,Imperial College London Du Cane Road,London,W120NN,UK

出　　处：《Journal of Innovative Optical Health Sciences》2014年第5期99-113,共15页创新光学健康科学杂志（英文）

基　　金：funding from the UK Biotechnology and Biological Sciences Research Council(BBSRCBB/H00713X/1);the UK Engineering and PhysicalSciences Research Council(EPSRC EP/IO2770X/1);the UK Technology Strategy Board Technology Award(CHBT/007/00030,EP/C54269X,inpartnership with Astra Zeneca,GE Healthcare,GSK,Kentech Instruments Ltd).

摘　　要：Fluorescence lifetime imaging(FLIM)is increasingly used to read out cellular autofluorescenceoriginating from the coenzyme NADH in the context of investigating cell metabolic state.Wepresent here an automated multiwell plate reading FLIM microscope optimized for UV illumi-nation with the goal of extending high content fluorescence lifetime asays to readouts ofmetabolism,We demonstrate its application to automated cellular autofluorescence lifetime imaging and discuss the key practical issues associated with its implementation.In particular,weillustrate its capability to read out the NADH-lifetime response of cells to metabolic modulators,thereby illustrating the potential of the instrument for cytotoxicity studies,assays for drugdiscovery and stratified medicine.

关 键 词：High content analysis FLIM NADH METABOLISM cisplatin. 

分 类 号：H31[语言文字—英语]