Deep tissue imaging by enhanced photon collection  

作　　者：Viera Crosignani Sohail Jahid Alexander Dvornikov Enrico Gratton 

机构地区：[1]Department of Biomedical Engineering University of California at IrvineIrvine,CA 92697-2715,USA

出　　处：《Journal of Innovative Optical Health Sciences》2014年第5期136-144,共9页创新光学健康科学杂志（英文）

基　　金：Funding was provided by National Institutes of Health P50 GM076516;by the National Center for Research Resources(5P41RR003155-27);the National Instit ute of General Medical Sciences(8 P41GM103540-27).

摘　　要：We have developed a two-photon fluorescence microscope capable of imaging up to 4mm inturbid media with micron resolution.The key feature of this instrument is the innovative de-tector,capable of collecting emission photons from a wider surface area of the sample thandetectors in traditional two-photon microscopes.This detection scheme is extremely efficient inthe collection of emitted photons scattered by turbid media which allows eight fold increase in theimaging depth when compared with conventional two-photon microscopes.Furthermore,thissystem also has in-depth fluorescence lifetime imaging microscopy(FLiM)imaging capabilitywhich increases image contrast.The detection scheme captures emission light in a transmissionconfiguration,making it extremely efficient for the detection of second harmonic generation(SHG)signals,which is generally forward propagating.Here we present imaging experiments oftissue phantoms and in vivo and ea vivo biological tissue performed with this microscope.

关 键 词：Deep tissue microscopy fluorescence SHG FLIM 

分 类 号：O43[机械工程—光学工程]