Correlation of in vivo tumor response and singlet oxygen luminescence detection in mTHPC-mediated photodynamic therapy  

作　　者：Brian C.Wilson Michael S.Patterson Buhong Li Mark T.Jarvi 

机构地区：[1]Department of Medical Biophysics University of Toronto/University Health Network Toronto,Ontario M5G 1L7,Canada [2]Juravinski Cancer Centre and McMaster University Hamilton,Ontario L8V 5C2,Canada [3]MOE Key Laboratory of OptoElectronic Science and Technology for Medicine Fujian Provincial Key Laboratory for Photonics Technology Fujian Normal University,Fuzhou,Fujian 350007,P.R.China

出　　处：《Journal of Innovative Optical Health Sciences》2015年第1期106-112,共7页创新光学健康科学杂志（英文）

基　　金：supported by the Canadian Cancer Society Research Institute (014245);the Fujian Provincial Natural Science Foundation (2011J06022).

摘　　要：Excited-state singlet axygen(^(1)O_(2)),generated during photodynamic therapy(PDT),is believed to be the primary cytotoxic agent with a number of clinically approved photosensitizers.Its relative concentration in cells or tissues can be measured directly through its near-infrared(NIR)luminescence emission,which has correlated well with in vitro cell and in triro normal skin treatment responses.Here,its correlation with the response of tumor tiss1e in vito is examined,using the photosensitizer meso-tetralydroxyphenylchlorin(mTHPC)in an animal model comprising luciferase-and green fluorescent protein(GFP)-transduced gliosarcoma grown in a dorsal window chamber.The change in the bioluminescence signal,imaged pre-treatment and at 2,5 and 9 d post treatment,was used as a quantitative measure of the tumor response,which was classified in individual tumors as"non","moderate"and"strong"in order to reduce the variance in the data.Plotting the bioluminescence-based response vs the^(1)O_(2)counts demonstrated clear correlation,indicating tha^(1)O_(2)luminescence provides a valid do-simetric technique for PDT in tumor tissue.

关 键 词：Photodynamice therapy singlet axygen lumninescence dosinetry BIOLUMINESCENCE 

分 类 号：R73[医药卫生—肿瘤]