Ultraviolet light A irradiation induces immunosuppression associated with the generation of reactive oxygen species in human neutrophils  

作　　者：Cunbo Li Xuechen Shi Mincai Chen Guangxue Xu Xinglei Su Pengchong Jiang Leiting Pan 

机构地区：[1]The Key Laboratory of Weak-Light Nonlinear Photonics Ministry of Education School of Physics and TEDA Applied Physics Institute Nankai University,Tianjin 300071,P.R.China [2]Department of Blood Transfusion PLA 307 Hospital,Beijing 100071,P.R.China [3]School of Life Sciences,Lanzhou University Lanzhou 730000,P.R.China [4]Shenzhen Key Laboratory of Micro-Nano Measuring and Imaging in Biomedical Optics College of Optoelectronic Engineering,Shenzhen University Shenzhen 518060,P.R.China

出　　处：《Journal of Innovative Optical Health Sciences》2016年第1期57-63,共7页创新光学健康科学杂志（英文）

基　　金：This work was supported by the National Natural Science Foundation of China(No.11204142),the National Basic Research Program of China (No.2013CB328702);International S&T Cooperation Program of China (2011DFA52870);the 111 Project(No.B07013),the National Science Fund for Talent Training in Basic Sciences(No.J1103208),the PCSIRT (IRT0149);the Social Development Fund of Ge'ermu Science and Technology Bureau and the State Key Laboratory of Medicinal Chemical Biology.

摘　　要：Ultraviolet blood irradiation has been used as a physical therapy to treat many nonspeci¯c diseases in clinics;however,the underlying mechanisms remain largely unclear.Neutrophils,the first line of host defense,play a crucial role in a variety of in°ammatory responses.In the present work,we investigated the effects of ultraviolet light A(UVA)on the immune functions of human neutrophils at the single-cell level by using an inverted°uorescence microscope.N-Formylmethionyl-leucyl-phenylalanine(FMLP),a classic physiological chemotactic peptide,was used to induce a series of immune responses in neutrophils in vitro.FMLP-induced calcium mobilization,migration,and phagocytosis in human neutrophils was significantly blocked after treatment with 365 nm UVA irradiation,demonstrating the immunosuppressive effects of UVA irradiation on neutrophils.Similar responses were also observed when the cells were pretreated with H2O2,a type of reactive oxygen species(ROS).Furthermore,UVA irradiation resulted in an increase in NAD(P)H,a member of host oxidative stress in cells.Taken together,our data indicate that UVA irradiation results in immunosuppression associated with the production of ROS in human neutrophils.

关 键 词：UVA irradiation reactive oxygen species NAD(P)H IMMUNOSUPPRESSION Human neutrophils 

分 类 号：R73[医药卫生—肿瘤]