Enhancing sensitivity of SERRS nanoprobes by modifying heptamethine cyanine-based reporter molecules  

作　　者：Yunfei Zhang Danqi Li Xingyu Zhou Xihui Gao Shengyuan Zhao Cong Li 

机构地区：[1]Key Laboratory of Smart Drug Delivery Ministry of Education&PLA,School of Pharmacy Fudan University,826 Zhangheng Rd.,Shanghai,201203,P.R.China [2]First Clinical College of Tongji Medical College Huazhong University of Science and Technology 13 Hangkong Rd.,Wuhan,Hubei,430030,P.R.China

出　　处：《Journal of Innovative Optical Health Sciences》2016年第4期85-93,共9页创新光学健康科学杂志（英文）

基　　金：supported by the National Basic Research Program of China(973 Program,2013CB932500);the National Natural Science Foundation of China(Nos 81371624,81571741);the Nanotechnology Program of Shanghai Science and Technology Committee(13NM1400400,15140901300);The innovation and entrepreneurship training program for college students in Huazhong University of Science&Technology(14A215).Yunfei Zhang and Danqi Li contributed equivalently to this work.

摘　　要：Surface enhanced resonance Raman scattering(SERRS)is a physical phenomenon that occurs when the energy of incident light is dose to that of electronic excitation of reporter molecules(RMs)attached on substrates.SERRS has showed great promise in healthcare applications such as tumor diagnosis,image guided tumor surgery and real-time evaluation of therapeutic response due to its ultra-sensitivity,manipulating convenience and easy acessibility.As the most widely used organic near-infrared(NIR)fuorophore,heptamethine cyanines possess the electronic ex-citation energy that is close to the plasmon absorption energy of the gold nano scafolds,which results in the extraordinary enhancement of the SERRS signal.However,the effect of hepta-methine cyanine structure and the gold nanoparticle morphology to the SERRS intensity are barely investigated.This work developed a series of SERRS nanoprobes in which two hepta-methine cyanine derivatives(IR783 and IR780)were used as the RM and three gold nanoparticles(nanorod,nanosphere and nanostar)were used as the substrates.Interestingly,even though IR780 and IR783 possess very similar chemical structure,SERRS signal produced by IR780 was determined as 14 times higher than that of IR783 when the RM concentration was6.5 × 10^(-6) M.In contrast,less than 4.0 fold SERRS signal intensity increase was measured by changing the substrate morphologies.Above experimental results indicate that finely tuning the chemical structure of the heptamethine cyanine could be a feasible way to develop robust SERRS probes to visualize tumor or guide tumor resection with high sensitivity and target to background ratio.

关 键 词：Surface enhanced resonance Raman scattering gold nanoparticles reporter molecules heptamethine cyanine. 

分 类 号：R73[医药卫生—肿瘤]