冠突散囊菌发酵鲜桑叶对其黄酮抑制α-葡萄糖苷酶活性的影响  

Effect of Eurotium cristatum Fermentation onα-Glucosidase Inhibitory Activity of Mulberry Leaf Flavonoids

作　　者：刘瑾如 邱卫华[1] LIU Jin-ru;QIU Wei-hua(State Key Laboratory of Biochemical Engineering,Institute of Process Engineering,Chinese Academy of Sciences,Beijing 100190,China;Department of Agricultural Engineering,College of Engineering,China Agricultural University,Beijing 100083,China)

机构地区：[1]中国科学院过程工程研究所生化工程国家重点实验室,北京100190 [2]中国农业大学工学院农业工程系,北京100083

出　　处：《食品研究与开发》2023年第4期203-208,共6页Food Research and Development

基　　金：建生鲜药创研基金项目(JSJC-20200104-056)。

摘　　要：为提高桑叶中黄酮类物质含量并改善其α-葡萄糖苷酶抑制活性,采用冠突散囊菌(Eurotium cristatum CY-1)进行鲜桑叶固态发酵的研究。研究结果表明,E.cristatum CY-1可以新鲜桑叶为发酵基质进行良好的生长。以30 g鲜桑叶(含水量76.92%)为发酵基质进行固态发酵时,第8天菌体生物量达到378.2 mg。随着发酵的进行,桑叶黄酮提取物(mulberry leaf flavonoids,MLF)的含量在第8天时升至最高值,为3.289 mg/g干桑叶,相对于未发酵桑叶提高了78.7%。MLF对α-葡萄糖苷酶的半数抑制浓度(IC_(50))随着发酵时间的延长而显著降低,其中经E.cristatum CY-1发酵10 d的桑叶MLF和未发酵桑叶的MLF的IC_(50)值分别为4.925μg/mL和25.995μg/mL。综上,冠突散囊菌的发酵不仅可以有效提高桑叶黄酮的含量,还可以提高其对α-葡萄糖苷酶的抑制效率。To enhance the flavonoids content in mulberry leaves and enhance theirα-glucosidase inhibitory activity,EurotiumcristatumCY-1 was used for the solid-state fermentation of freshmulberry leaves(ML).Using 30 g fresh ML(water content of 76.92%)as substrate for solid-fermentation,a maximum dry weight of 378.2 mg was obtained on day eight of fermentation.Moreover,the flavonoids content in fermented ML increased up to a maximum of 3.289 mg/g dry ML on day eight,which was 78.2%higher than that of unfermented ML.The half inhibition concentration(IC_(50))of flavonoidsα-glucosidase activity extracted from MLs decreased significantly when the duration of fermentation stage was increased.Notably,the inhibitory activity ofα-glucosidase from flavonoids extracted from ML fermented for 10 days and unfermented ML was 4.925μg/mL and 25.995μg/mL,respectively.In conclusion,this study showed that the fermentation of E.cristatum could both increase the content of ML flavonoids and also enhance theirα-glucosidase inhibitory activity.

关 键 词：鲜桑叶 冠突散囊菌 黄酮 Α-葡萄糖苷酶抑制剂 半数抑制浓度 

分 类 号：TQ464.8[化学工程—制药化工]