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作 者:王和寿 Wang Heshou(Ningde Inspection and Testing Centre for Agricultural Product Quality and Safety,Ningde 352100,Fujian,China)
机构地区:[1]宁德市农产品质量安全检验检测中心,福建宁德352100
出 处:《作物杂志》2022年第6期208-213,共6页Crops
基 金:福建省公益类科研院所基本科研专项(2019R1031-5)。
摘 要:为探究不同施 N 量对叶用甘薯营养品质的影响,及为制定科学的叶用甘薯施肥技术提供数据依据,选用叶用甘薯品种台农 71 和福菜薯 18 为材料,设氮素水平 0.00(CK)、87.50(T1)、175.00(T2)、262.50(T3)和 350.00g/m^(2)(T4),通过液质联用色谱仪和可见分光光度计分别测定样品中黄酮类化合物、可溶性蛋白和可溶性糖含量变化。检测发现,异槲皮苷含量最高,在福菜薯 18 中高达 74.43mg/kg。2 个品种均不含二氢槲皮素,其余的 5 种含量均较低。检测到的 6 种类黄酮与施 N 量呈极显著负相关关系(P<0.01),品种间没有差异。在施 N 量达到 262.50g/m^(2)(T3)时,福菜薯 18 总黄酮量达到最大,而施 N 量变化对台农 71 总黄酮量的影响不显著(P>0.05)。施 N 后,2 个品种的可溶性蛋白含量均显著低于未施 N 处理,可溶性蛋白含量与施 N 量呈显著负相关(P<0.01),在施 N 量达到 262.50g/m^(2)(T3)时,2 个品种可溶性蛋白含量均达到最大。可溶性糖含量与施 N 量呈正相关关系,但未达显著水平(P>0.05),而可溶性蛋白与可溶性糖含量之间呈显著负相关关系(P<0.05)。本试验发现,施 N 量为 262.50g/m^(2)时既能保证叶用甘薯的产量,又可以保证其保健品质。In order to explore the effects of different nitrogen application rates on the nutritional quality of sweet potato leaves and provide basis for formulating scientific fertilization technology, two kinds of sweet potato varieties, Tainong 71 and Fucaishu 18 were selected, and five nitrogen levels were set up, which were 0.00 (CK)87.50 (T1), 175.00 (T2), 262.50 (T3), and 350.00g/m^(2)(T4). The contents of flavonoids, soluble protein and soluble sugar in the samples were determined by liquid chromatography-mass spectrometry and visible spectrophotometer The results showed that the content of isoquercitrin was the highest among the seven flavonoids, which reached to 74.43mg/kg in Fucaishu 18, dihydroquercetin was found in both varieties, and the contents of five other flavonoids were relatively low. There were significant negative correlations between the six detected flavonoids and the amount of N applied (P < 0.01), and there was no difference between two varieties. When the amount of N application reached 262.50g/m^(2)(T3), the total flavone mass of Fucaishu 18 reached the maximum, and the change of N application rate was not significant for the total flavonoids of Tainong 71 (P > 0.05). After N application, the soluble protein content of the two varieties were significantly lower than that of the treatment without N application, and there was a significant negative correlation between the soluble protein content and N application rate (P < 0.01). Soluble protein amount per unit area reached the maximum when the nitrogen application rate reached 262.50g/m^(2)(T3). There was a positive correlation between soluble sugar content in leaves and N application rate, but it did not reach a significant level (P > 0.05), while there was a significant negative correlation between soluble protein and soluble sugar contents (P < 0.05). The results showed that when the N application rate was 262.50g/m^(2), it could not only ensure the yield of leaf sweet potato, but also its health quality.
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