转录组学在眼科研究中的应用  

Application of transcriptomics on ophthalmological research

作　　者：李玉婷 李妍[1] 李雅頔 黄悦 李浦一 胡竹林 Li Yuting;Li Yan;Li Yadi;Huang Yue;Li Puyi;Hu Zhulin(Department of Ophthalmology,Affiliated Hospital of Yunnan University,The Second People’s Hospital of Yunnan Province,Yunnan Eye Hospital,Fourth Affiliated Hospital of Kunming Medical University,Yunnan Eye Institute,Yunnan Clinical Medical Research Center of Eye Diseases,Yunnan Clinical Medical Center of Eye Diseases,Key Laboratory of Yunnan Province for the Prevention and Treatment of Ophthalmology,Kunming 650021,China)

机构地区：[1]云南大学附属医院眼科、云南省第二人民医院眼科、云南省眼科医院、昆明医科大学第四附属医院眼科、云南省眼科研究所、云南省眼部疾病临床医学研究中心、云南省眼病临床医学中心、云南省眼科疾病研究重点实验室,昆明650021

出　　处：《国际眼科纵览》2022年第5期391-396,共6页International Review of Ophthalmology

基　　金：云南省基础研究计划(昆医联合专项)(202001AY070001(-165));云南省基础研究专项(202201AT070073)。

摘　　要：转录组学是一门从分子机制角度研究某一机体组织的疾病发展过程的学科。在眼科疾病的转录组学研究中,角膜、房水、晶状体、视网膜等都富含了大量遗传信息,是转录组学理想的研究对象。转录组学测序技术通过序列杂交或基因测序等研究方式应用于微生物性角膜炎、先天性白内障、葡萄膜炎、糖尿病视网膜病变、年龄相关性黄斑变性、视网膜色素变性等眼科疾病的基础和临床研究中,为眼科疾病的发病机制及诊疗思路提供新的方向。Transcriptomics is a discipline that studies the disease process of a certain body tissue from the perspective of molecular mechanism.Transcriptomics sequencing technology has been widely used in research in various fields.In the transcriptomic study of ophthalmic diseases,cornea,aqueous humor,lens and retina are rich in a large amount of genetic information,which are ideal research objects of transcriptomics.Transcriptomics sequencing technology,through sequence hybridization or gene sequencing approach,applied in the laboratory and clinical researches on some ophthalmic diseases,such as microbial keratitis,congenital cataract,uveitis,diabetic retinopathy,age-related macular degeneration,retinitis pigmentosa,will provide a new direction for the pathogenesis,diagnosis and treatment of these diseases.

关 键 词：转录组学 RNA-seq技术 眼科疾病 

分 类 号：R77[医药卫生—眼科]