超声评价血管紧张素Ⅱ联合β-氨基丙腈诱导小鼠主动脉夹层模型主动脉形态与血流动力学变化特点  

Application value of ultrasound in evaluating the morphological and hemodynamic changes of aorta in mice model with aortic dissection induced by angiotensinogenⅡcombined withβ-aminopropionitrile

作　　者：黄冠男 牛会平[2] 武天娇 李亚楠[1] 赵艳琴[1] 薛丽丽[1] 谢迎新[1] 马冬[3] 赵雅培[1] Huang Guannan;Niu Huiping;Wu Tianjiao;Li Yanan;Zhao Yanqin;Xue Lili;Xie Yingxin;Ma Dong;Zhao Yapei(Department of Ultrasound,the East Branch of the Second Hospital of Hebei Medical University,Shijiazhuang 050035,China;Department of Endoscopy Center,the East Branch of the Second Hospital of Hebei Medical University,Shijiazhuang 050035,China;Key Laboratory of Neural and Vascular Biology,Hebei Medical University,Shijiazhuang 050017,China Key Laboratory of Neural and Vascular Biology,Hebei Medical University,Shijiazhuang 050017,China)

机构地区：[1]河北医科大学第二医院东院区超声科,石家庄050035 [2]河北医科大学第二医院东院区内镜中心,石家庄050035 [3]河北医科大学神经及血管生物学重点实验室,石家庄050017

出　　处：《中华超声影像学杂志》2022年第12期1084-1091,共8页Chinese Journal of Ultrasonography

摘　　要：目的:应用彩色多普勒超声(CDUS)观察血管紧张素Ⅱ(AngⅡ)联合β-氨基丙腈(BAPN)诱导小鼠主动脉夹层模型主动脉各段形态及血流动力学指标的变化特点。方法:选取20只健康状况良好的6~8周龄雄性C57BL/6小鼠,随机分为2组:模型组(n=10),AngⅡ联合BAPN腹腔注射建立小鼠主动脉夹层模型;对照组(n=10),腹腔注射生理盐水。常规记录小鼠体重、收缩压和舒张压,第42 d应用CDUS测量2组小鼠升主动脉、胸降主动脉和肾上主动脉的横截面内径,以及收缩期最高流速(PSV)、舒张期末期流速(EDV)、阻力指数(RI)、搏动指数(PI)、时间平均流速(TAMV)、心率、血流最大剪切率(SR)等。将主动脉自根部至肾动脉分叉部分取出,行HE染色观察主动脉壁病理变化。结果:①模型组与对照组体重、收缩压、舒张压、心率差异有统计学意义(均P<0.05);与对照组比较(0/10),模型组升主动脉夹层发生率(8/10)明显增高,差异有统计学意义(P<0.05);而胸降主动脉夹层发生率(4/10)、肾上主动脉夹层发生率(3/10)略增高,差异无统计学意义(均P>0.05)。②模型组升主动脉内径、PSV、EDV、TAMV、PI、SR明显高于对照组,差异有统计学意义(均P<0.05);两组间RI差异无统计学意义(P>0.05)。模型组胸降主动脉PSV、EDV、TAMV、PI、SR明显高于对照组,差异有统计学意义(均P<0.05);两组间内径、RI差异无统计学意义(均P>0.05)。模型组肾上主动脉PSV、TAMV、RI、PI、SR明显高于对照组,差异有统计学意义(均P<0.05);两组间内径、EDV差异无统计学意义(均P>0.05)。③模型组主动脉组织HE染色显示,主动脉明显扩张、不规则,管壁不均匀增厚;内皮细胞核染浅淡,部分内膜及中层断裂向外凸出形成夹层动脉瘤;外膜炎症细胞明显浸润。结论:超声可初步评价高血压合并主动脉夹层血流动力学改变,主动脉PSV、TAMV、PI和SR可能是早期预测高血压发生主动脉夹层的重要指标�Objective To observe the morphological and hemodynamics changes of aortic segments in mice with angiotensinogenⅡ(Ang II)combined withβ-aminopropionitrile(BAPN)induced-aortic dissection by color Doppler ultrasound(CDUS).Methods Twenty male mice of 6-8 weeks old C57BL/6 were randomly divided into two groups:the model group(n=10)was induced by intraperitoneal injection of AngⅡcombined with BAPN to establish mice model with aortic dissection;the control group(n=10)was intraperitoneally injected with normal saline.The body weight,systolic and diastolic blood pressure of the mice were routinely recorded.On the 42th day,CDUS was used to measure the indexes of ascending aorta(AoA),descending thoracic aorta(DAo)and suprarenal aorta(SAo)in both groups,including the inner diameter of the cross section,peak systolic velocity(PSV),the end diastolic velocity(EDV),the resistance index(RI),the pulsatility index(PI),time average mean velocity(TAMV),the heart rate(HR)and the maximal shear rate(SR).Then,the aortas were harvested from the root to the bifurcation of the renal artery.The pathological changes of the aortic wall were observed using hematoxylin-eosin(HE)staining.Results①There were statistically significant differences in body weight,systolic blood pressure,diastolic blood pressure and heart rate between the model group and the control group(all P<0.05).Compared with the control group(0/10),the incidence of the AoA dissection(8/10)in the model group was obviously higher,the difference was statistically significant(P<0.05);while the incidence of the DAo dissection(4/10)and the SAo dissection(3/10)in the model group was slightly higher,the differences were not statistically significant(all P>0.05).②Compared with the ascending aorta of the control group,the inner diameter,PSV,EDV,TAMV,PI and SR in the model group were significantly higher(all P<0.05),while RI showed no significant difference between the two groups(P>0.05).For the descending thoracic aorta,PSV,EDV,TAMV,PI and SR in model group were higher than those

关 键 词：超声检查 血管紧张素Ⅱ β-氨基丙腈 主动脉夹层 血流动力学 

分 类 号：R-332[医药卫生] R543.1