不同针刺方案对卒中后抑郁大鼠神经递质及相关炎性因子的影响  

Effects of different acupuncture schemes on neurotransmitters and related inflammatory factors in rats with post-stroke depression

作　　者：秦彦强[1,2] 董浩 孙迎春[1,2] 程先宽[1,2] 姚海江[1,2] QIN Yanqiang;DONG Hao;SUN Yingchun;CHENG Xiankuan;YAO Haijiang(Treatment Center of Traditional Chinese Medicine,Beijing Bo'ai Hospital,China Rehabilitation Research Center,Beijing 100068,China;Capital Medical University School of Rehabilitation Medicine,Beijing 100068,China;Beijing Massage Hospital,Beijing 100035,China)

机构地区：[1]中国康复研究中心北京博爱医院中医治疗中心,北京市100068 [2]首都医科大学康复医学院,北京市100068 [3]北京按摩医院,北京市100035

出　　处：《中国康复理论与实践》2023年第1期30-37,共8页Chinese Journal of Rehabilitation Theory and Practice

基　　金：中央级公益性科研院所基本科研业务费专项资金项目(No.2018CZ-3)。

摘　　要：目的探讨不同针刺方案对卒中后抑郁(PSD)大鼠行为学、神经递质及炎性相关因子的影响。方法健康雄性Sprague-Dawley大鼠72只,随机分为正常组、模型组、药物对照组、头针组、腹针组和联合针刺组,每组12只。模型组和各干预组采用Longa线栓法和慢性不可预知性温和应激(CUMS)法复合制备PSD模型。药物对照组予氟西汀灌胃,头针组针刺百会、印堂穴,腹针组针刺中脘、关元穴,联合针刺组针刺百会、印堂、中脘、关元穴,连续治疗21 d。分别于实验0 d(造模前)、7 d(CUMS前)、14 d(治疗前)、35 d(治疗后)进行Longa神经功能缺损评分、体质量、旷场试验、糖水偏好实验。实验35 d采用ELISA法检测血清5-羟色胺(5-HT)、多巴胺(DA)、去甲肾上腺素(NE)、干扰素-γ(INF-γ)、肿瘤坏死因子α(TNF-α)和巨噬细胞移动抑制因子(MIF)浓度。结果实验35 d时,药物对照组和联合针刺组神经功能缺损评分明显低于模型组(P<0.01),药物对照组、腹针组、联合针刺组体质量明显高于模型组(P<0.01),药物对照组、头针组、腹针组、联合针刺组水平跨格数、直立次数和糖水消耗量均高于模型组(P<0.05);药物对照组、头针组、腹针组、联合针刺组组间均无显著性差异(P>0.05)。药物对照组、头针组、腹针组和联合针刺组5-HT、DA、NE明显高于模型组(P<0.01),IFN-γ、TNF-α、MIF明显低于模型组(P<0.01)。联合针刺组5-HT高于头针组和腹针组,IFN-γ、TNF-α、MIF明显低于头针组和腹针组(P<0.01),DA、NE明显高于腹针组(P<0.01)。结论头针-腹针联合针刺方案可以改善PSD大鼠行为学症状,调节神经递质,调控炎性因子,效果与氟西汀相似。单纯使用头针或腹针也有较好疗效,可以作为次要选择。Objective To investigate the effects of different acupuncture schemes on behaviors,neurotransmitters and inflammationrelated factors in post-stroke depressed(PSD)rats.Methods A total of 72 healthy male Sprague-Dawley rats were randomly divided into normal group,model group,drug control group,scalp acupuncture group,abdominal acupuncture group and combined acupuncture group,12 rats in each group.The PSD model was prepared using a combination of middle cerebral artery occlusion and chronic unpredictable mild stimulation(CUMS)for the model and each intervention groups.The drug control group was administered fluoxetine,the scalp acupuncture group accepted acupuncture at Baihui(DU 20)and Yintang(EXHN3),while the abdominal acupuncture group at Zhongwan(RN 12)and Guanyuan(RN 4),the combined acupuncture group at all the four acupoints,for 21 days.They were assessed with Longa neurological function score,body mass,open-field test and sugar-water preference test on the 0(before modeling),the seventh(before CUMS),the 14th(before treatment)and the 35th day(after treatment).The levels of 5-hydroxytryptamine(5-HT),dopamine(DA),norepinephrine(NE),interferon-γ(INF-γ),tumor necrosis factor-α(TNF-α)and macrophage migration inhibitory factor(MIF)in the serum were determined with ELISA on the 35th day.Results The neurological function score was lower in the drug control group and the combined acupuncture group than in the model group(P<0.01)on the 35th day;while the body mass was higher in the drug control group,the abdominal acupuncture group and the combined acupuncture group than in the model group(P<0.01);and the number of horizontal span frames,the number of uprightness and the sugar water consumption were higher in the drug control group,the scalp acupuncture group,the abdominal acupuncture group and the combined acupuncture group than in the model group(P<0.05).There was no significant difference among the four intervention groups(P>0.05).The levels of 5-HT,DA and NE were higher in the four intervention groups than in the

关 键 词：卒中后抑郁 针刺 神经递质 炎性因子 大鼠 

分 类 号：R743.3[医药卫生—神经病学与精神病学]