宫颈组织PAX1甲基化定量检测诊断高级别宫颈癌前病变价值  

Efficacy of quantitative detection of paired boxed gene 1 methylation for screening high-grade cervical precancerous lesions

作　　者：李巧莉 马金枝 祝莉[1] LI Qiaoli;MA Jinzhi;ZHU Li(Sanmenxia Hospital of Yellow River,Sanmenxia,Henan Province,472000)

机构地区：[1]黄河三门峡医院,472000

出　　处：《中国计划生育学杂志》2023年第2期466-469,共4页Chinese Journal of Family Planning

摘　　要：目的:探讨宫颈组织配对盒家族基因1(PAX1)的甲基化定量检测对高级别宫颈癌前病变的临床诊断价值。方法:选取2021年6月-2022年6月本院妇科接受检查且确诊为高危亚型HPV病毒感染患者64例作为研究对象,均接受病理诊断并依据结果分为正常宫颈组(n=19)、低级别宫颈癌前病变组(n=21)、高级别宫颈癌前病变组(n=24),收集各组宫颈组织标本,采用焦磷酸测序法检测各样本PAX1基因的甲基化水平,对比各组HPV病毒阳性感染率及PAX1甲基化阳性率情况及宫颈刮片和宫颈组织中PAX1基因甲基化水平,采用受术者工作特征曲线(ROC)分析PAX1基因甲基化水平诊断高级别宫颈癌前病变价值。结果:随着宫颈癌前病变级别的升高,受检者高危HPV病毒DNA定量值(392.17±95.62、442.18±113.47、533.38±203.15)无差异(P=0.204),但HPV阳性率及PAX1甲基化阳性率正常宫颈组(36.8%、0)、低级别宫颈癌前病变组(66.7%、9.5%)、高级别宫颈癌前病变组(91.7%、62.5%)逐渐升高(均P<0.05):ROC曲线分析,PAX1基因甲基化水平诊断高级别宫颈癌前病变的曲线下面积为0.785,灵敏度89.5%、特异度80.5%。结论:PAX1甲基化定量检测方法可有效诊断高级别宫颈癌前病变。Objective: To investigate the value of quantitative detection of paired boxed gene 1(PAX1) methylation for diagnosing high-grade cervical precancerous lesions. Methods: From June 2021 to June 2022, a total of 64 patients with high-risk subtype HPV infection were selected as the study subjects. All the patients had received pathological diagnosis, and according to the results of pathological diagnosis, these patients were divided 19 cases with normal cervix in group A, 21 cases with low-grade cervical precancerous lesions in group B, and 24 cases with high-grade cervical precancerous lesions in group C. The cervical tissue samples of the patients in the three groups were collected, and the methylation level of PAX1 gene in the cervical tissue samples of the patients was detected by pyrosequencing method. The positive rate of HPV infection, and the positive rate and the level of PAX1 methylation in cervical tissue from cervical scrape of the patients were compared among the three groups. Receiver operating characteristic curve(ROC) was used to analyze the value of the level of PAX1 methylation in cervical tissue of the patients for diagnosing their high-grade cervical precancerous lesions. Results: There was no significant difference in the quantitative values of high-risk HPV DNA(392.17±95.62 vs. 442.18±113.47 vs. 533.38±203.15) of the patients among the three groups(P=0.204). The positive rates of HPV and PAX1 methylation(36.8% and 0) of the patients in group A, those(66.7% and 9.5%) of the patients in group B, and those(91.7% and 62.5%) of the patients in had increased gradually(all P<0.05). ROC curve analysis showed that the area under the curve, the sensitivity, and the specificity of PAX1 methylation level detection of the patients for diagnosing their high-grade cervical precancerous lesions were 0.785, 89.5%, and 80.5%, respectively. Conclusion: The methylation level of the women detected by quantitative ion of PAX1 methylation can effectively diagnose their high-grade cervical precancerous lesions.

关 键 词：高级别宫颈癌前病变 配对盒家族基因1 甲基化 焦磷酸测序 诊断效能 

分 类 号：R737.33[医药卫生—肿瘤]