双光子钙成像技术在非人灵长类动物大脑皮层神经元功能的研究现状  

Research status of two-photon calcium imaging of cortical neuronal functions in non-human primates

作　　者：孙伟铭 刘观秀[3,4] 董香丽 冯珍 马朝林[1,2] SUN Weiming;LIU Guanxiu;DONG Xiangli;FENG Zhen;MA Chaolin(College of Life Sciences,Nanchang University,Nanchang 330031,China;Institute of Life Sciences,Nanchang University,Nanchang 330031;Department of Rehabilitation Medicine,the First Affiliated Hospital of Nanchang University,Nanchang 330006;the First Clinical School,Nanchang University,Nanchang 330031;Department of Psychosomatic Medicine,the Second Affiliated Hospital of Nanchang University,Nanchang University,Nanchang 330006)

机构地区：[1]南昌大学生命科学学院,南昌330031 [2]南昌大学生命科学研究院,南昌330031 [3]南昌大学第一附属医院康复医学科,南昌330006 [4]南昌大学第一临床医学院,南昌330031 [5]南昌大学第二附属医院心身医学科,南昌330006

出　　处：《中国实验动物学报》2022年第7期949-954,共6页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然基金项目(31760276,31960171);江西省自然基金项目(20171BAB204019,20192ACB20022);江西省研究生创新专项资金项目(YC2021-B021)。

摘　　要：钙离子是生物体神经系统中重要的信使,可产生多种细胞信号,对神经元兴奋性的调控起着关键作用;钙离子在明确的细胞亚区发挥着高度特异性功能。钙信号间接反映神经元的活性,检测神经元钙信号对研究大脑皮层功能尤为重要。双光子钙成像技术对检测大脑皮层中的钙信号有独特的优势,可在体以单细胞分辨率实时观测皮层神经元的活动。研究非人灵长类动物的大脑皮层对提高人类神经系统的高级认知、精神疾病的治疗及推动康复医学的发展有重大意义。本文综述了双光子钙成像技术在非人灵长类动物皮质功能研究中的应用。Calcium ions are important messengers in the nervous system,which produce various cellular signals and play an important role in the regulation of neuronal excitability.Calcium ions in the clear cell area perform highly specific functions.Calcium signals indirectly reflect the activity of neurons,calcium signal detection in neurons is especially important to study cerebral cortex functions.Two-photon calcium imaging in the cerebral cortex has a unique advantage by revealing real-time in vivo activity of cortical neurons at the single cell resolution.This article reviews the application of two-photon calcium imaging in the study of cortical functions in non-human primates.

关 键 词：双光子钙成像技术 非人灵长类动物 大脑皮层 神经生物学 

分 类 号：Q95-33[生物学—动物学]