1株高耐性肠杆菌的筛选及对镉、砷同步钝化  

Inactivation of Cd and As by an Enterobacter Isolated from Cd and As Contaminated Farmland Soil

作　　者：尹雪斐 刘玉玲[1,2,3] 伍德 黄薪铭 张朴心 铁柏清 YIN Xue-fei;LIU Yu-ling;WU De;HUANG Xin-ming;ZHANG Pu-xin;TIE Bo-qing(College of Resources and Environment,Hunan Agricultural University,Changsha 410128,China;Hunan Engineering&Technology Research Center for Irrigation Water Purification,Changsha 410128,China;Key Laboratory of Southern Farmland Pollution Prevention and Control,Ministry of Agriculture and Rural Affairs,Changsha 410128,China)

机构地区：[1]湖南农业大学资源环境学院,长沙410128 [2]湖南省灌溉水源水质污染净化工程技术研究中心,长沙410128 [3]农业农村部南方产地污染防控重点实验室,长沙410128

出　　处：《环境科学》2023年第1期436-443,共8页Environmental Science

基　　金：湖南省高新技术产业科技创新引领计划项目(科技攻关类)(2020NK2001);国家重点研发计划项目(2017YFD0801505)。

摘　　要：从镉、砷复合污染农田土壤中筛选出1株具有硫酸盐还原功能的肠杆菌,通过去除率实验结合表征实验来探究其对镉、砷钝化机制.结果表明,经鉴定筛选出来的菌株M5为肠杆菌属(Enterobacter sp.),具有硫酸盐还原功能,对镉、砷最大耐受浓度为1.0 mmol·L^(-1)左右.在模拟体系中,菌株M5对镉去除率最大达94.13%,对砷去除率最大为27.26%. SEM-EDS和XRD的结果证实了Cd和As被固定成CdS和As_(2)S_(3),XPS结果表明该菌表面的羧基、羟基和酰胺基主要参与生物吸附.结果为微生物应用修复重金属及类金属污染土壤提供思路和理论依据.A strain of Enterobacter was screened from cadmium and arsenic contaminated farmland soil and its passivation mechanism of cadmium and arsenic were explored through removing performance and characterization experiments. The results showed that the screened strain M5 was identified as Enterobacter sp. with a sulfate-reduction function, and its maximum resistance concentration was approximately 1 mmol·L^(-1)to cadmium and arsenic. In the simulation system, the maximum removal efficiencies of cadmium and arsenic were 94.13% and 27.26% by strain M5, respectively. The results of SEM-EDS and XRD confirmed that Cd and As were fixed to CdS and As_(2)S_(3), and XPS results showed that carboxyl groups, hydroxyl groups, and amide groups on the surface of the bacteria were mainly involved in biological adsorption. These results can provide new ideas and a theoretical basis for microbial applications to soil remediations for heavy metal pollution.

关 键 词：肠杆菌 硫酸盐还原 镉(CD) 砷(As) 微生物成矿 

分 类 号：X171.5[环境科学与工程—环境科学]