基于QMEC分析的青藏高原不同类型冰川前缘地土壤微生物功能潜力  

QMEC-based Analysis of the Soil Microbial Functional Potentials across Different Tibetan Plateau Glacier Forelands

作　　者：张洁洁 Anders Priemé 陈显轲 周汉昌 张沁唯 庄绪亮[1,7] 秦翔 庄国强 马安周[1,6] ZHANG Jie-jie;Anders Priemé;CHEN Xian-ke;ZHOU Han-chang;ZHANG Qin-wei;ZHUANG Xu-liang;QIN Xiang;ZHUANG Guo-qiang;MA An-zhou(Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085,China;Sino-Danish College,University of Chinese Academy of Sciences,Beijing 101400,China;Sino-Danish Center for Education and Research,Beijing 101400,China;Department of Biology,University of Copenhagen,Universitetsparken 15,DK-2100,Copenhagen,Denmark;Center for Permafrost(CENPERM),University of Copenhagen,Øster Voldgade 10,DK-1350,Copenhagen,Denmark;College of Resources and Environment,University of Chinese Academy of Sciences,Beijing 101408,China;Institute of Tibetan Plateau Research,Chinese Academy of Sciences,Beijing 100101,China;Qilian Shan Station of Glaciology and Ecologic Environment,State Key Laboratory of Cryospheric Science,Northwest Institute of Eco-environment and Resources,Chinese Academy of Sciences,Lanzhou 730000,China)

机构地区：[1]中国科学院生态环境研究中心,北京100085 [2]中国科学院大学中丹学院,北京101400 [3]中国-丹麦科研教育中心,北京101400 [4]Department of Biology,University of Copenhagen,Universitetsparken 15,DK-2100,Copenhagen,Denmark [5]Center for Permafrost(CENPERM),University of Copenhagen,Øster Voldgade 10,DK-1350,Copenhagen,Denmark [6]中国科学院大学资源与环境学院,北京101408 [7]中国科学院青藏高原研究所,北京100101 [8]中国科学院西北生态环境资源研究院冰冻圈科学国家重点实验室,祁连山冰川与生态环境综合观测研究站,兰州730000

出　　处：《环境科学》2023年第1期512-519,共8页Environmental Science

基　　金：第二次青藏高原综合科学考察研究项目(2019QZKK0307,2019QZKK0402);国家重点研发计划项目(2018YFA0901204);中国科学院科技服务网络计划项目(KFJ-EW-STS-140)。

摘　　要：微生物通过多种功能代谢过程主导着因气候变暖裸露的冰川前缘地土壤元素的地球化学循环.以青藏高原的海洋型冰川、亚大陆型冰川和极大陆型冰川的前缘地土壤为研究对象,分析不同类型冰川前缘地土壤的微生物功能特征.依次选择玉龙冰川、天山乌鲁木齐1号冰川和老虎沟12号冰川作为三类冰川的典型代表,采用高通量功能基因芯片(QMEC)检测土壤微生物的功能基因特征.结果表明,在三类冰川前缘地土壤中,半纤维素降解基因和还原型乙酰辅酶A途径相关的碳固定基因丰度最高,三者主要的氮功能基因和氨化作用有关,磷、硫功能基因则主要与有机磷矿化过程和硫氧化过程相关.其中,水热条件较好的海洋型冰川的微生物功能基因的种类与丰度最高,其次为环境较为干燥的极大陆型冰川.三类冰川前缘地土壤的微生物功能基因结构的显著差异,证实了地理环境差异对微生物功能特征的影响,也为不同类型冰川前缘地土壤微生物的功能及其介导的元素地球化学循环研究提供了基础.Soil microorganisms dominate the biogeochemical cycles of elements in glacier forelands,which continue to expand due to the climate warming.We analyzed the soil microbial functional characteristics among three types of glacier forelands on the Tibetan Plateau:Yulong Glacier(Y),a temperate glacier;Tianshan Urumqi Glacier No.1(T),a sub-continental glacier;and Laohugou Glacier No.12(L),a continental glacier.Here,soil microbial functional genes were quantified using quantitative microbial element cycling technology(QMEC).We found that,in the three glacier forelands,the abundances of soil microbial functional genes related to hemicellulose degradation and reductive acetyl-CoA pathway were highest compared with other carbon-related functional genes.The main nitrogen cycling genes were involved in ammonification.The functional genes of the phosphorus cycle and sulfur cycle were related to organic phosphate mineralization and sulfur oxidation.Furthermore,the soils of the temperate glacier foreland with better hydrothermal conditions had the most complex microbial functional gene structure and the highest functional potentials,followed by those of the soils of continental glacier foreland with the driest environment.These significant differences in soil microbial functional genes among the three types of glacier forelands verified the impacts of geographic difference on microbial functional characteristics,as well as providing a basis for the study of soil microbial functions and biogeochemical cycles in glacier forelands.

关 键 词：青藏高原 冰川类型 前缘地土壤 微生物功能基因 高通量功能基因芯片(QMEC) 

分 类 号：X172[环境科学与工程—环境科学]