miR-144-3p对K562细胞增殖、周期及凋亡的影响  

Effects of miR-144-3p on Proliferation, Cell Cycle and Apoptosis of K562 Cells

作　　者：康娅 郝锦霞 韦素华 王梦昌[3] KANG Ya;HAO Jin-Xia;WEI Su-Hua;WANG Meng-Chang(Department of Health Information Services,The First Affiliated Hospital of Xi′an Jiaotong University;Xi′an Jiaotong University Hospital;Department of Hematology,The First Affiliated Hospital of Xi′an Jiaotong University,Xi′an 710061,Shaanxi Province,China)

机构地区：[1]西安交通大学第一附属医院医疗信息管理办公室 [2]西安交通大学医院 [3]西安交通大学第一附属医院血液内科,陕西西安710061

出　　处：《中国实验血液学杂志》2023年第1期76-80,共5页Journal of Experimental Hematology

基　　金：陕西省重点研发计划项目(2021SF-079)。

摘　　要：目的:研究mi R-144-3p对慢性髓性白血病急变期K562细胞增殖、周期和凋亡的影响。方法:体外培养K562细胞,通过转染试剂分别将模拟物阴性对照、mi R-144-3p模拟物、抑制物阴性对照、mi R-144-3p抑制物转染入K562细胞。将细胞分为空白对照、模拟物阴性对照、mi R-144-3p模拟物、抑制物阴性对照和mi R-144-3p抑制物共5组。采用CCK-8法检测细胞增殖,使用流式细胞术检测细胞周期和凋亡情况。结果:与空白对照、模拟物阴性对照组比较,mi R-144-3p模拟物组细胞增殖率明显减低(P<0.05),S期细胞明显增加(P<0.05),G1期细胞明显减少(P<0.05),细胞凋亡率明显升高(P<0.05);与空白对照、抑制物阴性对照组比较,mi R-144-3p抑制物组细胞增殖率明显升高(P<0.05),S期细胞明显减少(P<0.05),G1期细胞明显增加(P<0.05),细胞凋亡率明显下降(P<0.05)。结论:mi R-144-3p能够抑制K562细胞增殖、促进凋亡,影响细胞周期,将K562细胞阻滞在S期,表明mi R-144-3p参与了慢性髓性白血病急变期细胞周期活动。Objective: To investigate the effects of miR-144-3p on cell proliferation, cell cycle and apoptosis of blast phase chronic myelogenous leukemia(CML) K562 cells. Methods: K562 cells were cultured in vitro and mimics negative control, hsa-mi R-144-3p mimics, inhibitor negative control and mi R-144-3p inhibitor were respectively transfected into K562 cells with transfection reagents. The cells were divided into five groups including blank control, mimics negative control, mi R-144-3p mimics, inhibitor negative control and mi R-144-3p inhibitor. After transfection, the cell proliferation activity was detected by CCK-8 assay. The cell cycle distribution and apoptosis were detected by flow cytometry. Results: Compared with the blank control and mimics negative control groups, the proliferation rate of mi R-144-3p mimics group was significantly decreased(P<0.05), the proportion of S phase cells was markedly increased(P<0.05), while the proportion of G1 phase cells was obviously decreased(P<0.05), and the apoptosis rate was significantly increased(P<0.05). Compared with the blank control and inhibitor negative control groups, the proliferation rate of mi R-144-3p inhibitor group was obviously increased(P<0.05), the proportion of S phase cells was markedly decreased(P<0.05), while the proportion of G1 phase cells was obviously increased(P<0.05), and the apoptosis rate was significantly decreased(P<0.05). Conclusion: mi R-144-3p can inhibit the proliferation and promote apoptosis of K562 cells, affect the cell cycle, and block K562 cells in S phase, which indicates that mi R-144-3p is involved in the cell cycle activity of CML during blastic phase.

关 键 词：miR-144-3p K562细胞 细胞增殖 细胞凋亡 细胞周期 

分 类 号：R733.72[医药卫生—肿瘤]