金雀异黄素对淋巴瘤小鼠炎性易栓状态的体内干预及相关机制  

The In Vivo Intervention and Relative Mechanism of Genistein on the Inflammation and Thrombophilia in Lymphoma-Bearing Mice

作　　者：陈祉悦 施青青[2] 孙幸[2] 倪军[2] 吴蔚[2] 沈连军[2] 孙梅[2] 徐开林 顾健 顾昊[3] CHEN Zhi-Yue;SHI Qing-Qing;SUN Xin;NI Jun;WU Wei;SHEN Lian-Jun;SUN Mei;XU Kai-Lin;GU Jian;GU Hao(Xuzhou Medical University,Xuzhou 221000,Jiangsu Province,China;Department of Hematology,Northern People’s Hospital,Yangzhou 225001,Jiangsu Province,China;Department of Neurology,Brain Hospital Affiliated to Nanjing Medical University,Nanjing 210029,Jiangsu Province,China)

机构地区：[1]徐州医科大学,江苏徐州221000 [2]江苏省苏北人民医院血液科,江苏扬州225001 [3]南京医科大学脑科医院神经内科,江苏南京210029

出　　处：《中国实验血液学杂志》2023年第1期125-129,共5页Journal of Experimental Hematology

摘　　要：目的:探讨金雀异黄素(Genistein, GEN)对淋巴瘤鼠体内肿瘤相关炎性及易栓状态的干预及其机制。方法:将40只5-6周龄Balb/c雌性小鼠予以鼠源性Pro B细胞淋巴瘤细胞株38B9构建淋巴瘤荷瘤鼠模型,并随机分为对照组、荷瘤未干预、GEN干预和环磷酰胺(Cyclophosphamide, CTX)干预共4组,每组10只。采用组织病理学评估成瘤情况,观察成瘤情况,取肿瘤组织行HE及免疫组化染色。采用ELISA和FCM法等分别检测GEN和CTX干预后血浆中炎性细胞因子分泌水平和血栓指标的变化。采用免疫组化法检测CD19在瘤体小鼠瘤组织中的表达。结果:小鼠荷瘤14 d后均成瘤,瘤体组织中淋巴瘤细胞呈弥散分布,荷瘤鼠瘤体组织的CD19表达为阳性。荷瘤鼠体内炎性指标IL-6、NETs及CLEC-2和血栓指标TF、FIB及D-D均明显高于荷瘤前(均P<0.05)。GEN及CTX干预后上述指标水平均低于未干预组。GEN干预组CLEC-2和D-D水平明显低于CTX干预组(P<0.05)。结论:荷瘤淋巴瘤小鼠体内存在肿瘤相关炎性及易栓状态。GEN通过干预肿瘤炎性因子达到的抗炎和抗易栓作用优于CTX。Objective: To investigate the in vivo intervention and relative mechanism of Genistein(GEN) on tumor-associated inflammatory and tumor thrombophilia in lymphoma-bearing mice. Methods: Forty female Balb/c mice aged 5-6 weeks were injected with murine-derived Pro B-cell lymphoma cell line 38 B9 to establish a lymphoma mouse model, which was randomly divided into control group, tumor-bearing group, GEN drug intervention group and cyclophosphamide(CTX)drug intervention group. Histopathologic was used to evaluate the tumorigenesis. Tumor formation was observed, and tumor tissues were collected of HE and immunohistochemical staining. ELISA and flow cytometry were used to detect the expression of inflammatory factors and the changes of thrombus indices in plasma after intervention of GEN and Cyclophosphamide(CTX) respectively. Immunohistochemistry method was used to detect the expression of CD19 in tomor tissues of tummor bearing mice. Results: After 14 days of tumor bearing, the mice were tumorigenic. The lymphoma cells were diffusely distributed in the tumor tissue and the expression of CD19 in the tumor tissue was positive. The inflammatory factors such as IL-6, NETs and CLEC-2, and thrombotic indices such as TF, FIB and D-D in lymphoma-bearing mice were significantly higher than those before tumor-injection and lower than those after drug-intervention(all P<0.05). The levels of CLEC-2 and D-D in GEN group were significantly lower than those in CTX group(P<0.05). Conclusion: Tumor-associated inflammation and thrombophilia exist in lymphoma-bearing mice. GEN shows better anti-inflammatory and anti-thrombotic effects compared with CTX by interfering with tumor inflammatory factors.

关 键 词：金雀异黄素 淋巴瘤 荷瘤鼠 肿瘤炎性易栓症 

分 类 号：R733.1[医药卫生—肿瘤]