脑脊液中细胞因子IL-6、IL-10和CXCL-13的表达水平对淋巴瘤中枢神经系统浸润的诊断意义  

Diagnosis Significance of the Levels of Cytokines IL-6,IL-10 and CXCL-13 in Cerebrospinal Fluid for Central Nervous System Infiltration of Lymphoma

作　　者：刘丽欣 刘景华 王吉刚 佟丹江 孟广晗 李敏燕 周凡 LIU Li-Xin;LIU Jing-Hua;WANG Ji-Gang;TONG Dan-Jiang;MENG Guang-Han;LI Min-Yan;ZHOU Fan(Joint Postgraduate Training Base of Jinzhou Medical University and General Hospital of Northern Theater Command,Jinzhou 121000,Liaoning Province,China;Department of Hematology,General Hospital of Northern Theater Command,Shenyang 110016,Liaoning Province,China)

机构地区：[1]锦州医科大学北部战区总医院研究生培养基地,辽宁锦州121000 [2]北部战区总医院血液科,辽宁沈阳110016

出　　处：《中国实验血液学杂志》2023年第1期130-134,共5页Journal of Experimental Hematology

基　　金：中国博士后科学基金(2016M593026)。

摘　　要：目的:探讨细胞因子白介素-6(IL-6)、白介素-10(IL-10)和趋化因子-13(CXCL-13)在脑脊液中的表达水平对于淋巴瘤中枢神经系统浸润的诊断价值。方法:收集2020年7月至2021年7月在北部战区总医院就诊的30例淋巴瘤患者、10例急性淋巴细胞白血病患者的脑脊液,记录其脑脊液指标(包括压力、蛋白、潘氏试验、有核细胞计数、葡萄糖含量、氯含量),应用酶联免疫吸附实验检测细胞因子IL-6、IL-10和CXCL-13的水平。结果:根据中枢浸润与否分为中枢浸润组(CNSI组)和非中枢浸润组(non-CNSI组),CNSI组的IL-6、IL-10、IL-10/IL-6比值及CXCL-13表达水平均高于non-CNSI组,但仅IL-10/IL-6比值在两组之间差异具有统计学意义(P<0.05)。根据脑脊液蛋白是否增高分为蛋白增高组(n=14)和蛋白正常组(n=26),蛋白增高组的IL-6[(5.78±2.69)pg/ml]、CXCL-13水平[(0.83±0.59)pg/ml]明显高于蛋白正常组[IL-6(2.41±1.16)pg/ml、CXCL-13(0.38±0.18)pg/ml],差异具有统计学意义(P<0.05)。单独分析non-CNSI组(n=32)细胞因子的表达情况,蛋白增高组(n=12)的IL-6、IL-10、IL-10/IL-6比值和CXCL-13水平均高于蛋白正常组(n=20)。结论:淋巴瘤发生中枢神经系统浸润患者的脑脊液中细胞因子IL-6、IL-10和CXCL-13的水平高于未发生中枢浸润者,且脑脊液蛋白升高患者的IL-6、IL-10和CXCL-13因子水平高于脑脊液蛋白正常患者。Objective:To evaluate the diagnostic value of the expression levels of cytokines interleukin-6(IL-6),interleukin-10(IL-10)and chemokine(C-X-C motif)ligand-13(CXCL-13)in cerebrospinal fluid(CSF)for central nervous system infiltration of lymphoma.Methods:Forty patients diagnosed as lymphoma or acute lymphoblastic leukemia in General Hospital of Northern Theater Command from July 2020 to July 2021 were collected and recorded their CSF indexes,including pressure,protein,Pandy test,nucleated cell count,glucose and chlorine content in CSF.The levels of cytokines IL-6,IL-10 and CXCL-13 were detected by Enzyme-linked immunosorbent assay.Results:The patients were divided into CNSI(central nervous system infiltration)group and non-CNSI group,the average levels of IL-6,IL-10,CXCL-13 and IL-10/IL-6 ratio in CNSI group were higher than those in non-CNS group,but the difference of IL-10/IL-6 ratio between the two groups was statistically significant(P<0.05).Then the patients were divided into protein elevated(n=14)group and protein normal group(n=26),the levels of IL-6[(5.78±2.69)pg/ml]and CXCL-13[(0.83±0.59)pg/ml]in protein elevated group were significantly higher than those in the protein normal group[IL-6:(2.41±1.16)pg/ml;CXCL-13:(0.38±0.18)pg/ml](P<0.05).Further analysis of the expression levels of the cytokines in non-CNSI group(n=32),IL-6,IL-10,CXCL-13 level and IL-10/IL-6 ratio in the protein elevated group(n=12)w ere higher than those in the protein normal group(n=20),but the difference w as not statistically significant.Conclusion:The levels of IL-6,IL-10 and CXCL-13 in CSF of lymphoma patients w ith CNS infiltration w ere higher than those in non-CNS infiltration group,and those in patients w ith protein elevated group are higher than those in the protein normal group.

关 键 词：淋巴瘤中枢神经系统浸润 IL-6 IL-10 CXCL-13 

分 类 号：R733[医药卫生—肿瘤] R739.4[医药卫生—临床医学]