S100A16在食管鳞癌中的表达及其对肿瘤细胞迁移和侵袭能力的影响  

Expression of S100A16 in esophageal squamous cell carcinoma and the effects on the migration and invasion of esophageal carcinoma cells

作　　者：王海[1] 赵翠平[2] 周林艳 阚敬保 石永康[1] 查全斌 WANG Hai;ZHAO Cuiping;ZHOU Linyan;KAN Jingbao;SHI Yongkang;ZHA Quanbin(Department of Oncology,Jintan First People's Hospital,Jiangsu University,Changzhou 213002,China)

机构地区：[1]江苏大学附属常州市金坛第一人民医院肿瘤内科,江苏常州213002 [2]南京医科大学附属常州市第二人民医院老年医学科,213000 [3]江苏大学附属常州市金坛第一人民医院病理科,213002 [4]南京医科大学第一附属医院老年医学科,210000

出　　处：《临床肿瘤学杂志》2023年第1期23-29,共7页Chinese Clinical Oncology

基　　金：江苏省卫生健康委基金项目(Z2019057);江苏省干部保健课题(BJ21011);常州市卫生健康委基金项目(WZ201928)。

摘　　要：目的 探讨S100A16在食管鳞状细胞癌(ESCC)组织中的表达及其对ESCC细胞迁移、侵袭能力的影响。方法 利用生物信息学分析S100A16在ESCC中的表达趋势及生存情况,利用免疫组化进一步验证S100A16在ESCC患者癌组织中的表达,构建S100A16-overexpression和S100A16-siRNA细胞模型,采用细胞划痕实验及细胞侵袭实验观察S100A16对TE12细胞迁移能力及侵袭能力的影响。结果 GEO数据库中分析ESCC GSE26886芯片,发现S100A16在ESCC中表达上调;而在TCGA和GTEx数据库中分析发现,S100A16在ESCC中表达下调。利用基因表达谱动态分析(GEPIA)在线分析网站进行生存分析,结果显示S100A16对食管癌患者的总生存期及无病生存期均无显著影响(P>0.05)。S100A16在ESCC癌组织中的低表达率和高表达率分别为65.0%(52/80)、35.0%(28/80),S100A16在癌旁组织中的低表达率和高表达率分别为40.0%(32/80)、60.0%(48/80);S100A16在ESCC癌组织中低表达,与癌旁组织比较差异有统计学意义(P<0.05)。免疫组化结果显示,S100A16在ESCC患者癌组织的阳性表达总评分明显低于癌旁组织(3.60±3.54 vs. 5.94±3.69,P<0.01)。ESCC中的S100A16表达与年龄、性别、TNM分期无关(P>0.05),而与组织分化程度有关(P<0.001)。S100A16-siRNA组TE12细胞愈合能力和穿孔数量显著增强,均显著高于S100A16-overexpression组(P<0.001)。结论 S100A16在ESCC组织中低表达,高表达S100A16可抑制ESCC细胞的迁移及侵袭能力。Objective To examine the expression of S100A16 in esophageal squamous cell carcinoma(ESCC) specimens, and the effects on the migration and invasion ability of ESCC cells. Methods We examined the expression of S100A16 in ESCC samples by bioinformatics analysis and immunohistochemistry. In vitro,we constructed S100A16-overexpression and S100A16-siRNA cells to investigate the effect of S100A16 on the migration and invasion ability of TE12 cells. Results In GEO database, we found S100A16 was up-regulated in the GSE26886 microarray of ESCC, and TCGA and GTEx databases showed that S100A16 was down-regulated in ESCC. The survival analysis on gene expression profiling interactive analysis(GEPIA) website showed that S100A16 had no significant effect on the overall survival and disease-free survival of patients with esophageal cancer(P>0.05). In cancer tissues of ESCC, low expression of S100A16 accounted for 65.0%(52/80), high expression of S100A16 accounted for 35.0%(28/80). In adjacent tissues of ESCC, low expression of S100A16 accounted for 40.0%(32/80), and high expression of S100A16 accounted for 60.0%(48/80). S100A16 was low expressed in cancer tissues of ESCC, and the difference was statistically significant compared with adjacent tissues(P<0.05). Immunohistochemistry showed that the total score of positive expression of S100A16 in cancer tissues of patients with ESCC was significantly lower than that of adjacent tissues(3.60±3.54 vs. 5.94±3.69,P<0.01). The expression of S100A16 in ESCC tissues was not related to the age, sex and TNM stage(P>0.05), but related to differentiation grade(P<0.001). Compared with the S100A16-overexpression group, the healing ability and the number of perforations of TE12 cells in the S100A16-siRNA group were significantly enhanced(P<0.001). Conclusion The expression of S100A16 was lower in ESCC tissues, and the up-regulation of S100A16 inhibits the migration and invasion ability of ESCC cells.

关 键 词：食管鳞状细胞癌 S100A16基因 迁移 侵袭 

分 类 号：R735.1[医药卫生—肿瘤]