羟基自由基快速杀灭刺激隐核虫及其对大黄鱼肠道结构的影响  

·OH inactivation of Cryptocaryon irritans and its effect on intestinal morphology of Larimichthys crocea

作　　者：卞永宁 徐茹 高蕊 魏源[2] 方宏达 白敏冬 BIAN Yongning;XU Ru;GAO Rui;WEI Yuan;FANG Hongda;BAI Mindong(Fujian Key Laboratory of Coastal Pollution Prevention and Control,College of the Environment and Ecology,Xiamen University,Xiamen 361005,China;State Key Laboratory of Environmental Criteria and Risk Assessment,Chinese Research Academy of Environmental Sciences,Beijing 100012,China;ZKTH Environmental Technology Co.,Ltd.,Xiamen 361005,China)

机构地区：[1]福建省海岸带污染防控重点实验室、厦门大学环境与生态学院,福建厦门361005 [2]中国环境科学研究院环境基准与风险评估国家重点实验室,北京100012 [3]中科同恒环境科技有限公司,福建厦门361005

出　　处：《应用海洋学学报》2023年第1期56-65,共10页Journal of Applied Oceanography

基　　金：国家自然科学基金联合基金重点资助项目(U1905212);国家重大科研仪器研制项目资助项目(62127806)。

摘　　要：刺激隐核虫(Cryptocaryon irritans)的大规模暴发严重危及大黄鱼养殖业的健康发展,造成重大经济损失。在福建省宁德市三都澳海域大黄鱼(Larimichthys crocea)养殖基地,采用大气压强电离放电高效生成的羟基自由基(·OH)溶液输入到海水管路中,快速(0.53 mg/L,6 s)杀灭(100%)刺激隐核虫,再排入净水池中储存作为大黄鱼的养殖水。经·OH处理后的海水养殖大黄鱼,鱼肠道组织结构完整、肠绒毛长且密,大黄鱼存活率100%;净水池养殖用水中弧菌、总大肠菌群、耐热大肠菌群均未检出,且细菌总数为0 CFU/mL,水质符合《海水水质标准》第二类标准,三溴甲烷含量为7.84μg/L,远低于《生活饮用水卫生标准》的限值100μg/L,溴酸盐、三氯甲烷等均未检出,海洋生态安全。Large-scale outbreak of Cryptocaryon irritans during cultivations of Larimichthys crocea is a serious threat to the health mariculture,The outbreak caused significant economic losses in China.In experiments at a L.crocea breeding base in Sanduao Sea,Ningde City,Fujian Province,the hydroxyl radicals(·OH)produced by a strong ionization discharge were injected into the pipeline to inactivate Cryptocaryon irritans(100%)rapidly at concentrations of 0.53 mg/L in 6 s and then the solution was discharged into clean water pool stored as L.crocea farming water.L.crocea cultured in·OH treated seawater had a complete intestinal structure with long and dense intestinal villi and a 100%survival rate.Vibrio,total coliforms and heat-resistant coliforms were not detected in waters from the clean water pool,and the total bacteria was 0 CFU/mL.The water quality met the requirements of Class II by the National Seawater Quality Standard.Moreover,bromate,chloroform,etc.were not detected,and tribromomethane was only 7.84μg/L,which was much lower than the limitation of 100μg/L set by the Sanitary Standards for Drinking Water.Thus,the method can be used safely for marine ecology.

关 键 词：海洋环境科学 羟基自由基 快速杀灭 大黄鱼 刺激隐核虫 肠道结构 

分 类 号：P76[天文地球—海洋科学]