肝细胞癌组织p53凋亡刺激蛋白2表达及临床意义  

Expression of ASPP2 in hepatocellular carcinoma and its clinicopathological significance

作　　者：沈凯[1] 刘彤[1] SHEN Kai;LIU Tong(Department of General Surgery,Beijing Tongren Hospital Affiliated to Capital Medical University,Beijing 100730,China)

机构地区：[1]首都医科大学附属北京同仁医院普通外科,100730

出　　处：《浙江医学》2023年第2期121-124,149,共5页Zhejiang Medical Journal

摘　　要：目的探讨肝细胞癌组织p53凋亡刺激蛋白2(ASPP2)表达及临床意义。方法选取2016年6月至2019年6月在首都医科大学附属北京同仁医院接受手术切除治疗的80例肝细胞癌患者为研究对象,收集所有患者的肝细胞癌组织及癌旁正常组织,比较两种组织ASPP2表达水平;分析肝细胞癌组织ASPP2蛋白表达与临床病理特征、预后以及与X连锁凋亡抑制蛋白(XIAP)、p53蛋白表达的关系。结果肝细胞癌组织ASPP2 mRNA表达水平、蛋白高表达率和蛋白表达评分均明显低于癌旁正常组织(均P<0.05)。肝细胞癌组织ASPP2蛋白表达与病理分期、临床分期、脉管浸润等有关(均P<0.05),与性别、年龄、HBV感染、肝硬化、甲胎蛋白水平、肿瘤数量、包膜侵犯等均无关(均P>0.05)。ASPP2蛋白高表达患者累积无病生存率和累积5年总生存率均明显高于ASPP2蛋白低表达患者(均P<0.05)。ASPP2蛋白高表达的肝细胞癌组织XIAP蛋白表达水平明显低于ASPP2蛋白低表达的肝细胞癌组织,而p53蛋白表达水平明显高于ASPP2蛋白低表达的肝细胞癌组织,差异均有统计学意义(均P<0.05)。结论肝细胞癌组织ASPP2低表达与病理进展、预后不良有关,其作用机制可能与调控XIAP、p53有关。Objective To explore the expression of apoptosis-stimulating of p53 protein2(ASPP2)in hepatocellular carcinoma and its clinicopathological significance.Methods A total of 80 patients with hepa-tocellular carcinoma who underwent surgical resection in Beijing Tongren Hospital Affiliated to Capital Medical University from June 2015 to June 2018were enrolled in the study.The expression levels of ASPP2 mRNA and protein in cancer tissues and corresponding adjacent tissues were detected with qRT-PCR and immunohistochemistry,respectively.The relationship of ASPP2 m RNA expression with clinicopathological features and prognosis of patients was analyzed;and the correlation of ASPP2 expression with Xlinked inhibitor of apoptosis protein(XIAP)and p53 was also analyzed.Results The expression levels of ASPP2 m RNA and protein,the high expression rate in hepatocellular carcinoma tissues was lower than those in corresponding adjacent tissues(all P<0.05).The high expression of ASPP2 protein in hepatocellular carcinoma tissues was correlated with pathological grade,clinical stage and vascular invasion(all P<0.05);not correlated with gender,age,HBV infection,cirrhosis,tumor numbers,membrane infiltration and alpha-fetoprotein levels(all P>0.05).The accumulate disease free survival and 5-year overall survival in patients with high expression of ASPP2 protein was higher than those with low ASPP2(both P<0.05).The expression of XIAP protein was lower and the p53 protein was higher in patients with high ASPP2 expression than those with low ASPP2expression(both P<0.05).Conclusion The decreased expression of ASPP2 in hepatocellular carcinoma is correlated with pathological progress and poor prognosis,which are associated with the regulation of the XIAP and p53 protein expression.

关 键 词：肝细胞癌 p53凋亡刺激蛋白2 X连锁凋亡抑制蛋白 P53 预后 

分 类 号：R735.7[医药卫生—肿瘤]