浓缩生长因子用于比格犬成熟恒牙牙髓再生的实验研究  

An experimental study of concentrated growth factor on the regeneration of mature permanent dental pulp tissue in Beagle dogs

作　　者：王静[1] 林华洁 殷金萍 张筱娅 孟伟涛 WANG Jing;LIN Huajie;YIN Jinping;ZHANG Xiaoya;MENG Weitao(Department of Stomatology,The First Affiliated Hospital of Zhengzhou University,450000,China)

机构地区：[1]郑州大学第一附属医院口腔科,450000

出　　处：《实用口腔医学杂志》2023年第1期25-29,共5页Journal of Practical Stomatology

基　　金：河南省医学科技攻关计划省部共建项目(编号:SB201903006)。

摘　　要：目的:探讨浓缩生长因子(CGF)结合牙髓血运重建术在比格犬成熟恒牙牙髓再生中的作用。方法:选择6只18月龄比格犬的36颗上颌门齿,分为4组:阳性对照组不干预,阴性对照组建立根尖周炎并牙根吸收模型后不治疗,血运重建组建立同样模型后行牙髓血运重建术,血运重建加置CGF膜组建立同样模型后置CGF膜行牙髓血运重建术。术后拍摄X线片,HE染色观察各组牙组织学变化。结果:比格犬实验模型建立成功。血运重建组和血运重建加置CGF膜组根尖周阴影缩小,根尖部矿化组织中可见骨陷窝,但后者矿化组织更致密。结论:CGF结合牙髓血运重建术有促进犬成熟恒牙根尖周炎愈合和牙根发育的作用。Objective:To investigate the effects of concentrated growth factor(CGF)combined with revascularization on pulp regeneration of mature permanent teeth of Beagle dogs.Methods:36 maxillary incisors from 618-month-old beagle dogs were included and divided into 4 groups.The teeth in positive control group were not intervened.The teeth in negative group were used for the establishment of periapical periodontitis and root resorption models(PR)without other treatment.The teeth in revascularization group were used as the same PR models and treated with revascularization(PRR).The teeth in revascularization combined with CGF group were used as the same models and treated with revascularization combined with CGF(PRRC).X-ray apical radiographs were taken after operation,and the histological changes of the experimental teeth in various groups were observed by HE staining.Results:The experimental models of Beagle dogs were established successfully.In revascularization group and revascularization combined with CGF group,periapical shadows were reduced,and bone lacuna could be seen in the mineralized tissue of the apical area,but the mineralized tissue in the group of revascularization combined with CGF was more dense.Conclusion:CGF combined with revascularization can promote the healing of periapical tissue with periodontitis and can promote the root development of mature permanent teeth of beagle dogs.

关 键 词：浓缩生长因子 根尖周炎 牙根吸收 成熟恒牙 牙髓再生 

分 类 号：R781.3[医药卫生—口腔医学]