不同浓度葡萄糖对人牙髓细胞增殖凋亡及矿化的影响  

The effects of different concentrations of glucose on the proliferation,apoptosis and mineralization of human dental pulp cells

作　　者：韩永付 李国芳 裴蓉 马鹏涛 HAN Yongfu;LI Guofang;PEI Rong;MA Pengtao(Department of Stomatology,Children As Hospital of Zhengzhou University,Henan Children's Hospital,Zhengzhou Children's Hospital,450000,China)

机构地区：[1]郑州大学附属儿童医院,河南省儿童医院,郑州儿童医院口腔科,450000

出　　处：《实用口腔医学杂志》2023年第1期35-39,共5页Journal of Practical Stomatology

基　　金：河南省2019年科技发展计划(编号:192102310078)。

摘　　要：目的:探讨人牙髓细胞(hDPCs)在不同浓度葡萄糖作用下,细胞增殖凋亡及矿化情况。方法:体外培养的第4代hDPCs分为正常组、高糖1组和高糖2组,分别用含葡萄糖浓度为0、5.5、40和50 mmol/L的培养基培养,21 d后CCK-8实验检测细胞存活率,流式细胞仪检测细胞凋亡率,qRT-PCR检测DMP1和DSPP mRNA相对表达量;茜素红染色观察矿化结节情况,试剂盒检测MDA含量及SOD和ALP活性,Wester blot检测Bcl-2、Bax及DSPP和DMP1蛋白相对表达量。结果:正常组矿化结节明显,其余2组矿化结节少。正常组细胞存活率、SOD和ALP活性、DMP1和DSPP mRNA相对表达量、Bcl-2以及DMP1和DSPP蛋白相对表达量均高于高糖1组(P<0.05)和高糖2组(P<0.05),而细胞凋亡率、MDA含量、Bax蛋白相对表达量均低于高糖1组(P<0.05)和高糖2组(P<0.05),以上指标差异在高糖2组比1组更明显(P<0.05)。结论:高糖抑制人牙髓细胞增殖和矿化,可能是高糖增强氧化应激反应引起。Objective:To investigate the proliferation,apoptosis and mineralization of human dental pulp cells(hDPCs)treated with different concentrations of glucose.Methods:In vitro cultured hDPCs of passage 4 were divided into normal group,high glucose 1 group and high glucose 2 group and cultured in the medium with glucose concentration of 0,5.5,40 and 50 mmol/L respectively for 21 days.Then,CCK-8 assay was used to detect cell survival rate,cell apoptosis rate was detected by flow cytometry,and mRNA relative expression levels of DMP1 and DSPP were detected by qRT-PCR.The mineralized nodules were observed by alizarin red staining,the content of MDA,SOD and ALP was detected by relative test kits,and the relative expression levels of Bcl-2,Bax and DSPP and DMP1 were detected by Western blot.Results:The nodules of mineralization were obvious in the normal group,but fewer in the other 2 groups.Cell viability,SOD and ALP activity,mRNA relative expression levels of DMP1 and DSPP,Bcl-2,DMP1 and DSPP relative protein expression level of normal group were higher than those of high glucose 1 group(P<0.05)and high glucose 2 group(P<0.05),while cell apoptosis rate,the concent of MDA and the relative expression level of Bax protein of normal group were lower than those of high glucose 1 group(P<0.05)and high glucose 2 group(P<0.05),the changes of each index in high glucose group 2 were the most significant(P<0.05).Conclusion:High glucose concentration inhibits the proliferation and mineralization of hDPCs by oxidative stress.

关 键 词：人牙髓细胞 高糖 增殖 凋亡 矿化 

分 类 号：R783.3[医药卫生—口腔医学]