过表达miR-509对人舌鳞癌细胞SCC15纳武单抗敏感性的影响及机制研究  

The effects of miR-509 overexpression on navumab sensitivity of human tongue squamous cell SCC15 and its mechanism

作　　者：潘睿 韩炜 卢娜 PAN Rui;HAN Wei;LU Na(Department of Stomatology,Qinhuangdao Military Hospital,066000,China;Department of Function,Baoding Third Central Hospital;Department of Orthodontics,Affiliated Hospital of North China University of Science and Technology,Tangshan)

机构地区：[1]秦皇岛市军工医院口腔科,066000 [2]保定市第三中心医院功能科 [3]华北理工大学附属医院口腔正畸修复科

出　　处：《实用口腔医学杂志》2023年第1期93-97,共5页Journal of Practical Stomatology

基　　金：河北省2020年度医学科学研究课题(编号:20200133)。

摘　　要：目的:探讨miR-509对人舌鳞癌细胞SCC15纳武单抗敏感性的影响及机制。方法:纳入40例舌鳞癌患者的癌组织及癌旁组织,PCR法检测miR-509水平。取0~80μmol/L纳武单抗处理后以及分别转染miR-509 mimic和mimic NC的SCC15细胞,PCR法检测miR-509水平、流式细胞仪检测细胞凋亡率、蛋白印迹法检测凋亡相关蛋白。结果:癌组织中miR-509水平低于癌旁组织。细胞存活率随纳武单抗浓度的增加而降低(P<0.05)。与mimic-NC组比较,miR-509 mimic组miR-509表达水平、细胞凋亡率、活化的半胱氨酸天冬氨酸蛋白酶3(cleaved caspase3)以及Bcl-2相关X蛋白(Bax)蛋白表达升高,B淋巴细胞瘤-2基因(Bcl-2)蛋白表达降低(P<0.05)。结论:过表达miR-509增强SCC15细胞对纳武单抗的敏感性,可能是通过激活促凋亡蛋白表达,抑制抗凋亡蛋白表达发挥调控作用。Objective:To investigate the effects of miR-509 on the sensitivity of human tongue squamous cell carcinoma(TSCC)SCC15 cells to navulumab and its mechanism.Methods:The level of miR-509 in carcinoma tissues and paracancer tissues of 40 TSCC patients was detected by PCR.The SCC15 cells were treated with navumab of 0-80μmol/L and transfected with miR-509 mimic and mimic NC respectively,then the level of miR-509 was detected by PCR,cell apoptosis rate was detected by flow cytometry and apoptosis-related proteins were detected by Western blot.Results:The level of miR-509 in cancer tissues was lower than that in paracancer tissues.The survival rate of SCC15 cells was decreased with the increase of navumab concentration(P<0.05).Compared with mimic-NC group,the miR-509 expression level,cell apoptosis rate,activated cysteine caspase3 and Bcl-2 associated X protein(Bax)protein expression were increased in miR-509 mimic group,whele the protein expression of B lymphocytoma-2 gene(Bcl-2)was decreased(P<0.05).Conclusion:Overexpression of miR-509 enhances the sensitivity of SCC15 cells to navumab,which may play a regulatory role by activating the expression of pro-apoptotic proteins and inhibiting the expression of anti-apoptotic proteins.

关 键 词：舌鳞癌 纳武单抗 miR-509 

分 类 号：R739.8[医药卫生—肿瘤]