Transcriptome Sequencing and Analysis of Agaricus blazei Mycelia Under Cadmium Stress  

镉胁迫下姬松茸菌株转录组测序及分析

作　　者：ZHANG Yu-hui MA Xin-wang XIA Zhi-lan HUANG Min-feng SU Rong-rong 张宇慧;马鑫旺;夏志兰;黄民凤;苏荣荣(湖南省农业产业化发展服务中心,湖南长沙410006;湖南农业大学,湖南长沙410125;贵州黔西南喀斯特区域发展研究院,贵州兴义562400)

机构地区：[1]Hunan Agricultural Industrialization Development Service Center,Changsha 410006,PRC [2]Hunan Agricultural University,Changsha 410125,PRC [3]Southwest Guizhou Institute of Karst Regional Development,Xingyi 562400,PRC

出　　处：《Agricultural Science & Technology》2022年第4期54-64,共11页农业科学与技术（英文版）

基　　金：湖南省科技创新计划(2018JJ2166)。

摘　　要：To explore the mechanism of Agaricus blazei Murrill enrichment of the heavy metal cadmium,we employed Illumina high-throughput sequencing to analyze the transcriptomes of A.blazei mycelia treated with and without exogenous cadmium addition,and then the differentially expressed genes(DEGs)of the strains with high and low cadmium enrichment between the control and cadmium treatment were screened out.The results showed that the DEGs were mainly involved in steroid biosynthesis,antibiotic biosynthesis,protein processing in endoplasmic reticulum,glutathione metabolism and other pathways.Carbon metabolism and glutathione metabolism may play an important role in the response of A.blazei mycelium to cadmium stress.为探明姬松茸菌株对重金属镉的富集机理,以前期筛选出的镉高、低富集姬松茸菌株(J11、J4)为材料进行2 mg/L的镉胁迫处理,以未添加外源镉作对照,采用Illumina高通量测序技术对其菌丝样品进行转录组测序分析。结果表明:差异表达基因主要富集在类固醇生物合成、抗生素的生物合成、碳代谢、谷胱甘肽代谢、内质网的蛋白质加工、甲烷代谢、淀粉和蔗糖的代谢等通路;其中,类固醇生物合成、抗生素的生物合成途径上调;甲烷代谢、淀粉和蔗糖代谢、碳代谢、谷胱甘肽、乙醛酸和二羧酸等代谢途径下调。分析认为碳代谢、谷胱甘肽代谢可能在姬松茸菌株响应镉胁迫方面起重要的作用。

关 键 词：Agaricus blazei Murrill Cadmium stress Transcriptome sequencing GO analysis 

分 类 号：X173[环境科学与工程—环境科学] S646[农业科学—蔬菜学]