生长激素作用通路、调控机制及生物学活性测定研究进展  

Research progress on the action pathways,regulation mechanism and biological activity measurement of growth hormone

作　　者：张孝明 李晶[1] 梁成罡[1] ZHANG Xiao-ming;LI Jing;LIANG Cheng-gang(National Institutes for Food and Drug Control,NHC Key Laboratory of Research on Quality and Standardization of Biotech Products,Beijing 102629,China)

机构地区：[1]中国食品药品检定研究院、国家卫生健康委员会生物技术产品检定方法及其标准化重点实验室,北京102629

出　　处：《药物分析杂志》2022年第11期1865-1876,共12页Chinese Journal of Pharmaceutical Analysis

基　　金：中国食品药品检定研究院中青年发展研究基金(2022A1)。

摘　　要：生长激素是由脑垂体前叶嗜酸性细胞分泌的1种单一肽链的蛋白质激素,是人出生后促生长最重要的激素。生长激素通过与细胞表面特异性受体结合,激活非受体酪氨酸激酶JAK2及Src家族激酶的下游信号通路发挥生物学效应。深入了解生长激素作用机制是其生物学活性测定研究的基础。本文综述了近年来生长激素相关研究进展,包括生长激素与受体作用机制,生长激素与受体结合后激活的STATs、PI3K-Akt和MEK-Erk等信号通路,以及生长激素作用通路调控机制,并对生长激素活性测定方法研究进行了总结和展望,以期为生长激素药物生物学活性质量控制研究提供参考。Growth hormone is a protein hormone with a single peptide chain produced and released by the anterior pituitary,which is the most important hormone for growth promotion after birth.In order to exert biological effects,growth hormone first binds to a specific receptor on the cell surface,and then activates the down stream signaling pathways of the non-receptor tyrosine kinase JAK2 and Src family kinases.An in-depth understanding of the mechanism of action of growth hormone is required for its biological activity measurement.In this paper,we reviewed the recent research progress on growth hormone,including the mechanism of action of growth hormone receptor,the activation of STATs,PI3 K-Akt and MEK-Erk signaling pathways by the binding of growth hormone to the receptor,and the regulatory mechanism of growth hormone action pathways.We also summarized and prospected the research on the determination methods of growth hormone activity,in order to provide a reference for the quality control of the biological activity of growth hormone drugs.

关 键 词：生长激素 细胞信号通路 分子作用机制 生物学活性 质量控制 非受体酪氨酸激酶 生长激素受体 C-FOS基因 胰岛素样生长因子-1 信号转导及转录激活因子 

分 类 号：R917[医药卫生—药物分析学]