TSG-6对多房棘球蚴感染小鼠早期肝纤维化的影响  

Effect of TSG-6 on early liver fibrosis in Mice infected by echinococcus multilocularis

作　　者：卜媛媛 宋艳鹏 李琛 姜慧娇 刘程豪 乔世源 陈雪玲 吴向未[1] BU Yuanyuan;SONG Yanpeng;LI Chen;JIANG Huijiao;LIU Chenghao;QIAO Shiyuan;CHEN Xueling;WU Xiangwei(The First Affiliated Hospital,School of Medicine,Shihezi University,Shihezi,Xinjiang 832008,China;Department of Immunology,School of Medicine,Shihezi University,Shihezi,Xinjiang 832008,China)

机构地区：[1]石河子大学医学院第一附属医院,新疆石河子823008 [2]石河子大学医学院免疫学教研室,新疆石河子832008

出　　处：《石河子大学学报（自然科学版）》2022年第6期758-764,共7页Journal of Shihezi University(Natural Science)

基　　金：国家自然科学基金项目(81760570,81760371);新疆生产建设兵团科技发展专项项目(2018CB017,2019AB031)。

摘　　要：目的 探究TSG-6对多房棘球蚴感染模型小鼠早期肝纤维化的影响。方法 将20只肝脏已感染多房棘球蚴20d的C57BL/6小鼠随机分为模型组、TSG-6治疗组每组10只;另外设置对照组10只。TSG-6治疗组每三天经腹腔注射重组鼠TSG-6蛋白(rmTSG-6) 0.5μg/25μLPBS,对照组和模型组每三天经腹腔注射等量PBS。治疗30 d后处死各组小鼠,取各组小鼠血清,多房棘球蚴病灶周围肝组织以及对照组正常肝组织制作组织切片。ELISA检测各组小鼠血清中ALT及AST的含量、HE和Masson染色观察各组小鼠肝组织病理改变及胶原含量、免疫组化和Western blotting检测各组小鼠肝组织纤维化标志物α-SMA、CollagenI、CollagenIII的表达。结果 与模型组相比,经TSG-6治疗后各组小鼠血清中ALT、AST的含量减少,肝组织炎性细胞浸润与胶原纤维沉积减少,纤维化标志物α-SMA、CollagenI、CollagenIII的表达降低。结论 TSG-6抑制了多房棘球蚴感染小鼠早期肝纤维化。Objective To investigate the effect of TSG-6 on Liver fibrosis in mice infected with echinococcus multilocularis. Methods twenty C57 BL/6 mice infected with echinococcus multiloculae were randomly divided into model group and TSG-6 treatment group with 10 mice in each group. Another 10 were set as control group. The TSG-6 treatment group was intraperitoneally injected with recombinant mouse TSG-6 protein(rmTSG-6)0.5μg/25μLPBS every three days, and the control group and model group were intraperitoneally injected with the same amount of PBS every three days.Mice in each group were executed after 30 days of treatment, and the serum of mice in each group, liver tissue around echinococcus multilocularis and normal liver tissue in the control group were taken for tissue section.The content of ALT and AST in serum of mice in each group were detected by ELISA, Pathological changes of liver tissue and collagen content of mice in each group were observed by HE and Masson staining, and the expressions of α-SMA, CollagenI and CollagenIII in liver tissues were detected by immunohistochemistry and Western blotting. Results Compared to the model group, after TSG-6 treatment, the levels of ALT and AST in serum of each group were decreased, inflammatory cell infiltration and collagen fiber deposition in liver tissue were decreased, and the expressions of fibrosis markers α-SMA, CollagenI and CollagenIII were decreased.Conclusion TSG-6 can inhibit early Liver fibrosis in mice infected with echinococcus multilocularis.

关 键 词：多房棘球蚴 TSG-6 肝纤维化 

分 类 号：R575.2[医药卫生—消化系统]