“土茯苓-薏苡仁”药对治疗骨性关节炎的网络药理学分析  

Analysis of "Smilacis Glabrae Rhizoma-Coicis Semen" for the treatment of osteoarthritis based on network pharmacology

作　　者：王强[1] 于霞 张立清[3] 李纯璞[1] 张建[1] 魏开斌[1] WANG Qiang;YU Xia;ZHANG Liqing;LI Chunpu;ZHANG Jian;WEI Kaibin(Department of Joint Mobility Medicine,Tai'an Central Hospital,Tai'an Central Hospital Affiliated to Qingdao University,Taishan Medical and Nursing Center,Shandong Province,Tai'an271000,China;Department of Nuclear Medicine,Tai'an Central Hospital,Tai'an Central Hospital Affiliated to Qingdao University,Taishan Medical and Nursing Center,Shandong Province,Tai'an271000,China;Department of Orthopedics,Tai'an Rongjun Hospital of Shandong Province,Tai'an271000,China)

机构地区：[1]山东省泰安市中心医院青岛大学附属泰安市中心医院泰山医养中心关节运动医学科,山东泰安271000 [2]山东省泰安市中心医院青岛大学附属泰安市中心医院泰山医养中心核医学科,山东泰安271000 [3]山东省泰安荣军医院骨科,山东泰安271000

出　　处：《中国当代医药》2023年第5期14-18,F0003,F0004,共7页China Modern Medicine

基　　金：山东省老年医学学会科技攻关计划项目(LKJGG2021W121);山东省中医药科技项目(2021Z018);山东省泰安市科技创新发展项目(2021NS274)。

摘　　要：目的研究探讨“土茯苓-薏苡仁”药对治疗骨性关节炎的关键靶点以及作用机制。方法通过中药系统药理学分析平台(TCMSP)检索并筛选“土茯苓-薏苡仁”主要有效活性成分及其作用靶点,通过GeneCards数据库、OMIM数据库、PharmGkb数据库、TTD数据库、DrugBank数据库检索收集“骨性关节炎”的相关靶点,制作Venn图获取“土茯苓-薏苡仁”药对与骨性关节炎的共同靶点,运用Cytoscape软件构建“中药活性成分-骨性关节炎-靶点-疾病”网络,利用STRING数据库构建蛋白相互作用网络。最后运用R语言进行基因本体论(GO)功能富集分析和京都基因与基因组百科全书(KEGG)通路富集分析。结果中药“土茯苓-薏苡仁”对骨性关节炎有治疗作用的活性成分共19个(槲皮素等);发挥治疗作用的关键靶点共81个(丝氨酸/苏氨酸蛋白激酶1等);发挥疗效干预的生物学进程主要有对脂多糖的反应、对细胞外刺激的反应等;发挥重要作用的生物学通路主要有脂质和动脉粥样硬化通路、白细胞介素-17(IL-17)信号通路、肿瘤坏死因子(TNF)信号通路等。结论“土茯苓-薏苡仁”药对能够通过多成分、多靶点、多通路治疗骨性关节炎,为后续进一步研究和开展实验工作提供了思路和理论依据。Objective To explore the key target and mechanism of"Smilacis Glabrae Rhizoma-Coicis Semen"for the treatment of osteoarthritis.Methods Traditional Chinese Medicine System Pharmacology Analysis Platform was used to search and screen the main active components and targets of Smilacis Glabrae Rhizoma-Coicis Semen.The related targets of"osteoarthritis"were collected by GeneCards database,OMIM database,PharmGkb database,TTD database and DrugBank database.Venn diagram was made to obtain the common target of"Smilacis Glabrae Rhizoma-Coicis Semen"and osteoarthritis diease.Cytoscape software was used to construct the network of"Active Component-Osteoarthritis-Target-Disease".STRING platform was used to construct the protein-protein interaction network.Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)were carried out to perform functional enrichment analysis with R language.Results There were 19 active components(Quercetin,etc.)in the treatment of osteoarthritis,and 81 key targets in Chinese medcine"Smilacis Glabrae Rhizoma-Coicis Semen".Response to lipopolysaccharide and response to extracellular stimulus were the main biological processes for the occurrence and progression of osteoarthritis disease.The key pathways include lipid and atherosclerosis,interleukin-17(IL-17)signaling pathway,and tumor necrosis factor(TNF)signaling pathway,etc.Conclusion The effect of Chinese medicine on osteoarthritis may be realized through multi-components,multi-targets and multi-channels.

关 键 词：土茯苓 薏苡仁 骨性关节炎 网络药理学 作用机制 

分 类 号：R285[医药卫生—中药学]