一株降解园林绿化废弃物的毛头鬼伞的鉴定与固体产酶发酵条件优化  

Identification of a Coprinus comatus Degrading Landscaping Waste and Optimization of Enzymatic Fermentation Conditions

作　　者：徐东 何建清 葛彦宏 刘海鑫 韩振 刘林峰 XU Dong;HE Jianqing;GE Yanhong;LIU Hanxin;HAN Zhen;LIU Linfeng(Plant Science College,Tibet Agriculture&Animal Husbandry University,Nyingchi Tibet,860000 China)

机构地区：[1]西藏农牧学院植物科学学院,西藏林芝860000

出　　处：《高原农业》2023年第1期50-57,81,共9页Journal of Plateau Agriculture

基　　金：西藏自治区重点科技项目(XZ202001ZY0019N);国家自然科学基金项目(31560001);西藏农牧学院研究生创新计划项目(YJS2022-49,YJS2022-45)。

摘　　要：本研究以试验前期筛选到的一株野生产木质纤维素酶活性的食用菌为材料,根据形态学与rDNA-ITS序列对该食用菌进行鉴定。以园林废弃物粉为主要原料,采用单因素试验及正交试验对固体产酶条件进行优化。结果表明:试验食用菌菌株为毛头鬼伞Coprinus comatus。单因素试验证明:氮源、初始pH、温度和接种量为降解木质纤维素的显著影响因子,正交试验确定毛头鬼伞产羧甲基纤维素酶活性最优条件为:pH为7、温度25℃、接种量10%、碳氮比为15:1。该研究结果为园林废弃物木质纤维素的降解提供了良好的菌种资源,同时也为后续园林废弃物木质纤维素降解方面的研究积累了相关数据。In this study,a wild lignocellulase producing edible fungus selected in the early stage of the experiment was identified according to the combination of morphology and ITS biology.Using garden waste powder as the main raw material,solid single factor test and orthogonal test were used to optimize the condition of enzyme production.The results showed that the strain was preliminarily identified as Coprinus comatus by morphological and molecular biological characteristics.Single factor test showed that nitrogen source,initial pH,temperature and inoculum size were significant influencing factors for lignocellulose degradation.Orthogonal test determined that the optimal conditions for carboxymethyl cellulase production were pH 7,temperature 25℃,inoculum size 10%and C/N ratio 15:1.The research results provided a good strain resource for the degradation of lignocellulose from garden waste,and also accumulate relevant data for the subsequent research on lignocellulose from garden waste.

关 键 词：毛头鬼伞 固体发酵 木质纤维素 园林废弃物 酶活性 

分 类 号：S646[农业科学—蔬菜学]