M6A去甲基化酶FTO在脑胶质瘤中的表达及其与病人预后的关系  

Expression of m6A demethylase FTO in glioma tissues and its relationship with patients'prognoses

作　　者：张丽娜[1] 王凯[1] 范国锋 秦虎[1] ZHANG Li-na;WANG Kai;FAN Guo-feng;QIN Hu(Department of Neurosurgery,The First Affiliated Hospital of Xinjiang Medical University,Urumqi 841100,China)

机构地区：[1]新疆医科大学第一附属医院神经外科,乌鲁木齐841100

出　　处：《中国临床神经外科杂志》2023年第1期34-37,共4页Chinese Journal of Clinical Neurosurgery

基　　金：新疆维吾尔自治区卫生计生委青年科技人才专项科研项目(wjwy201818)。

摘　　要：目的探讨6-甲基腺嘌呤(m6A)去甲基化酶肥胖相关蛋白(FTO)在脑胶质瘤中的表达及其与病人生存预后的关系。方法选取2015年1月至2017年8月手术切除的脑胶质瘤标本84例(WHOⅡ级24例,Ⅲ级14例,Ⅳ级46例)和颅脑损伤内减压术中获取的非肿瘤脑组织40例为对照。免疫印迹法和免疫组化法检测组织FTO表达水平。胶质瘤病人随访截止2022年8月,计算总生存期。结果免疫组化染色显示,WHO分级Ⅱ、Ⅲ、Ⅳ级脑胶质瘤组织FTO高表达率分别为20.83%(5/24)、50.00%(7/14)、73.91%(34/46),均明显高于非肿瘤脑组织[10%(4/40);P<0.05];而且,WHO分级Ⅳ级胶质瘤FTO过表达率明显高于Ⅱ级胶质瘤(P<0.05)。免疫印迹法检查结果显示脑胶质瘤组织FTO蛋白表达水平明显高于非肿瘤脑组织(P<0.05)。截止随访结束,脑胶质瘤死亡30例,其中高表达组死亡18例,低表达组死亡12例。多因素logistic回归分析显示,FTO高表达是胶质瘤预后不良的独立危险因素(OR=3.794;95%CI 1.164~5.108;P<0.001)。生存曲线分析显示,FTO高表达组中位总体生存期较低表达组明显缩短(P<0.001)。结论脑胶质瘤组织m6A去甲基化酶FTO呈高表达,与病人不良生存预后有关。Objective To investigate the expression of 6-methyladenine(m6A)demethylase obesity-related protein(FTO)in glioma tissues and its relationship with patients'survival prognoses.Methods The expression of FTO was detected in glioma tissues obtained from 84 glioma patients who underwent surgery from January 2015 to August 2017(24 cases of WHO gradeⅡ,14 cases of WHO gradeⅢ,46 cases ofⅣ)and in non-tumoRbrain tissue obtained from 40 patients with traumatic brain injury who underwent decompression by western blotting and immunohistochemistry,respectively.The glioma patients were followed up until August 2022,and the overall survival time was calculated.Results Immunohistochemical staining showed that the high expression rates of FTO in glioma tissue of WHO gradeⅡ,Ⅲ,andⅣwere 20.83%(5/24),50.00%(7/14)and 73.91%(34/46),respectively,which were significantly higheRthan that[10%(4/40)]in non-tumoRbrain tissue(P<0.05).In addition,the high expression rate of FTO in WHO gradeⅣgliomas was significantly higheRthan that in gradeⅡgliomas(P<0.05).Western blotting showed that the expression level of FTO protein in glioma tissues was significantly higheRthan that in non-tumoRbrain tissues(P<0.05).By the end of follow-up,30 glioma patients died,including 18 patients with high expression of FTO and 12 with low expression.Multivariate logistic regression analysis showed that high expression of FTO was an independent risk factoRfoRpooRprognosis of glioma patients(OR=3.794;95%CI 1.164~5.108;P<0.001).Survival curve analysis showed that the median overall survival time was significantly shorteRin the patients with high FTO expression than the patients with low FTO expression(P<0.001).Conclusions The m6A demethylase FTO is highly expressed in glioma tissues,which is associated with a pooRsurvival prognosis of glioma patients.

关 键 词：脑胶质瘤 去甲基化酶 肥胖相关蛋白 生存预后 

分 类 号：R739.41[医药卫生—肿瘤] Q786[医药卫生—临床医学]