碱性成纤维细胞生长因子/壳聚糖衍生物/胶原温敏型复合水凝胶促大鼠牙周组织再生的研究  

Basic fibroblast growth factor/chitosan derivatives/collagen composite thermosensitive hydrogel promotes periodontal tissue regeneration in rats

作　　者：谭仲娟 罗圆圆 杨莉[1] Tan Zhongjuan;Luo Yuanyuan;Yang Li(Dept.of Endodontics,Affiliated Sto‐matology Hospital of Guangzhou Medical University,Guangdong Engineering Research Center of Oral Restoration and Reconstruction,Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine,Guangzhou 510182,China;Dept.of Stomatology,Liaocheng People’s Hospital,Liaocheng 252020,China)

机构地区：[1]广州医科大学附属口腔医院牙体牙髓科·广东省口腔组织修复与重建工程技术研究中心·广州市口腔再生医学基础与应用研究重点实验室,广州510182 [2]聊城市人民医院口腔科,聊城252020

出　　处：《华西口腔医学杂志》2023年第1期21-28,共8页West China Journal of Stomatology

基　　金：广州市科技计划项目(20180310044)。

摘　　要：目的 探讨不同种壳聚糖衍生物温敏型复合水凝胶作为支架材料应用于牙周组织工程的可行性。方法制备磺化壳聚糖(SCS)、磷酸化壳聚糖(PCS)及磷酸化磺化壳聚糖(PSCS) 3种具有不同生物学特性的壳聚糖衍生物,构建3种碱性成纤维细胞生长因子(bFGF)/壳聚糖衍生物/胶原温敏型复合水凝胶。选取20只雄性wistar大鼠,大鼠双侧上颌第一磨牙近中建立三壁骨袋,随机分为空白对照组、空白凝胶组、bFGF/SCS/胶原温敏型复合水凝胶组、bFGF/PCS/胶原温敏型复合水凝胶组、bFGF/PSCS/胶原温敏型复合水凝胶组,术后6周处死大鼠,采集标本,进行大体、苏木精-伊红染色、Masson染色观察。结果 术后6周,3种bFGF/壳聚糖衍生物/胶原温敏型复合水凝胶组与空白对照组在相对牙槽骨高度比值、相对上皮根向下移比及牙周组织再生分级计数等方面的差异均具有统计学意义(P<0.05)。结论 bFGF/壳聚糖衍生物/胶原温敏型复合水凝胶在牙周组织工程邻域具有良好的应用前景。Objective To investigate the feasibility of different thermosensitive composite hydrogels from chitosan derivatives as scaffold materials for periodontal tissue engineering. Methods Three chitosan derivatives with different biological characteristics were prepared, namely, sulfonated chitosan(SCS), phosphorylated chitosan(PCS), and phosphorylated sulfonated chitosan(PSCS). Three thermosensitive composite hydrogels were constructed using basic fibroblast growth factor(bFGF), the chitosan derivatives, and collagen. Twenty male Wistar rats were randomly divided into control group, blank group, bFGF/SCS/collagen composite thermosensitive hydrogel group, bFGF/PCS/collagen composite thermosensitive hydrogel group, and bFGF/PSCS/collagen composite thermosensitive hydrogel group. Then, threewall intrabony defects were established. The defects were treated with the different kinds of thermosensitive composite hydrogels. After 6 weeks of surgery, the animals were killed, and specimens were collected. Then, gross observation, hematoxylin-eosin staining, and Masson staining were performed. Results The b FGF/chitosan derivatives/collagen composite thermosensitive hydrogel groups and the control group had statistical differences in the relative alveolar bone height, relative epithelial down growth and grading count score of periodontal tissue regeneration(P<0.05). Conclusion b FGF/chitosan derivatives/collagen composite thermosensitive hydrogels have good application prospects in periodontal tissue engineering.

关 键 词：水凝胶 壳聚糖衍生物 碱性成纤维生长因子 胶原 牙周组织 再生 

分 类 号：R781.4[医药卫生—口腔医学]