右美托咪定对食管癌Eca-109细胞LINC00982表达及生物学行为的影响  

Effects of dexmedetomidine on LINC00982 expression and biological behavior of esophageal cancer Eca-109 cells

作　　者：奚高原 丁成智 孟睿 周晓蕾[3] 周俊辉[1] 钟巍 孟宪慧 XI Gaoyuan;DING Chengzhi;MENG Rui;ZHOU Xiaolei;ZHOU Junhui;ZHONG Wei;MENG Xianhui(Department of Anesthesiology,Chest Hospital Affiliated,Zhengzhou University,Zhengzhou 450008;Department of Thoracic Surgery,Chest Hospital Affiliated,Zhengzhou University,Zhengzhou 450008;Department of Respiratory and Critical Care Medicine,Chest Hospital Affiliated,Zhengzhou University,Zhengzhou 450008)

机构地区：[1]郑州大学附属胸科医院麻醉科,郑州450008 [2]郑州大学附属胸科医院胸外科,郑州450008 [3]郑州大学附属胸科医院呼吸与危重症医学科,郑州450008

出　　处：《郑州大学学报（医学版）》2023年第1期13-18,共6页Journal of Zhengzhou University(Medical Sciences)

基　　金：河南省科技攻关项目(202102310036)。

摘　　要：目的:探讨右美托咪定对食管癌Eca-109细胞LINC00982表达及生物学行为的影响及其可能作用机制。方法:以0、25、50、100μg/L右美托咪定处理的Eca-109细胞分别为对照组和右美托咪定低、中、高浓度组。pcDNA、pcDNA-LINC00982分别转染至Eca-109细胞,作为pcDNA和pcDNA-LINC00982组;si-NC、si-LINC00982分别转染至Eca-109细胞后加入100μg/L右美托咪定处理24 h,作为右美托咪定+si-NC组和右美托咪定+si-LINC00982组。分别应用CCK-8法、流式细胞术、平板克隆形成实验、划痕和Transwell实验检测各组细胞增殖、凋亡、克隆形成、迁移及侵袭情况;采用Western blot检测各组细胞Bax、E-cadherin、Bcl-2和N-cadherin蛋白的表达。采用qRT-PCR检测各组细胞LINC00982的表达。结果:与对照组比较,右美托咪定低、中、高浓度组Eca-109细胞增殖抑制率、细胞凋亡率、Bax和E-cadherin蛋白以及LINC00982表达水平升高,细胞克隆形成数、迁移及侵袭细胞数减少,划痕愈合率和Bcl-2、N-cadherin蛋白表达水平降低(P均<0.05);与pcDNA组比较,pcDNA-LINC00982组Eca-109细胞增殖抑制率、细胞凋亡率、Bax和E-cadherin蛋白表达水平升高,细胞克隆形成数、迁移及侵袭细胞数减少,划痕愈合率和Bcl-2、N-cadherin蛋白表达水平降低(P均<0.05);与右美托咪定+si-NC组比较,右美托咪定+si-LINC00982组Eca-109细胞增殖抑制率、细胞凋亡率和Bax、E-cadherin蛋白表达水平降低,细胞克隆形成数、迁移及侵袭细胞数增多,划痕愈合率和Bcl-2、N-cadherin蛋白表达水平升高(P均<0.05)。结论:右美托咪定可能通过上调LINC00982的表达而抑制食管癌细胞增殖、迁移及侵袭,并促进细胞凋亡。Aim:To explore the effects and mechanism of dexmedetomidine on LINC00982 expression and biological behavior of esophageal cancer Eca-109 cells.Methods:Eca-109 cells were treated with dexmedetomidine at 0,25,50 and 100 μg/L as the control group and dexmedetomidine low, medium and high concentrations groups, respectively.PcDNA and pcDNA-LINC00982 were transfected into Eca-109 cells as pcDNA group and pcDNA-LINC00982 group, respectively.The si-NC and si-LINC00982 were transfected into Eca-109 cells and treated with 100 μg/L dexmedetomidine for 24 hours, as dexmedetomidine+si-NC group and dexmedetomidine+si-LINC00982 group, respectively.The cell proliferation, apoptosis, cloning, migration and invasion were detected by CCK-8 assay, flow cytometry, plate cloning assay, wound healing assay and Transwell assay, respectively.Western blot was used to detect the expression of Bax, E-cadherin, Bcl-2 and N-cadherin proteins.The expression level of LINC00982 in each group was detected by qRT-PCR.Results:Compared with the control group, the proliferation inhibition rate, apoptosis rate, and the protein expression levels of Bax, E-cadherin and LINC00982 of Eca-109 cells in dexmedetomidine low, medium and high concentration groups were increased, the number of cell clones, migration and invasion cells was decreased, the wound healing rate, and the protein expression levels of Bcl-2 and N-cadherin were decreased(all P<0.05).Compared with pcDNA group, the proliferation inhibition rate, the rate of apoptosis, the protein expression levels of Bax and E-cadherin in the pcDNA-LINC00982 group were increased, and the number of cell clone, migration and invasion cells was decreased, and the wound healing rate and the protein expression levels of Bcl-2 and N-cadherin were decreased(all P<0.05).Compared with the dexmedetomidine+si-NC group, the proliferation inhibition rate, the rate of apoptosis, Bax and E-cadherin protein expression levels of Eca109 cells in the dexmedetomidine+LINC00982 group were decreased, the numbers of cell clones

关 键 词：右美托咪定 LINC00982 细胞增殖 细胞迁移 细胞侵袭 细胞凋亡 ECA-109细胞 

分 类 号：R735.1[医药卫生—肿瘤]