布比卡因对肝癌细胞Hep3B增殖、迁移及侵袭能力的影响  

Effects of bupivacaine on proliferation, migration and invasion abilities of liver carcinoma cells Hep3B

作　　者：孙婷 王震 黄素霞 崔明珠[2] 杨扬 SUN Ting;WANG Zhen;HUANG Suxia;CUI Mingzhu;YANG Yang(Department of Anesthesiology,Zhumadian Central Hospital,Zhumadian,Henan 463000;Department of Anesthesia and Perioperative Medicine,Henan Provincial People′s Hospital,Zhengzhou 450003)

机构地区：[1]驻马店市中心医院麻醉科,河南驻马店463000 [2]河南省人民医院麻醉与围术期医学科,郑州450003

出　　处：《郑州大学学报（医学版）》2023年第1期93-97,共5页Journal of Zhengzhou University(Medical Sciences)

基　　金：河南省医学科技攻关计划联合共建项目(LHGJ20191723)。

摘　　要：目的:探讨布比卡因对肝癌细胞Hep3B增殖、迁移及侵袭能力的影响及其可能作用机制。方法:体外培养人肝癌细胞Hep3B,分为空白对照组、100μmol/L布比卡因组、200μmol/L布比卡因组、400μmol/L布比卡因组,miR-阴性对照(NC)组、miR-143-3p组,抗miR-NC+400μmol/L布比卡因组、抗miR-143-3p+400μmol/L布比卡因组;分别采用MTT法、流式细胞仪、Transwell实验检测细胞增殖能力、细胞周期、迁移及侵袭能力;qRT-PCR法检测miR-143-3p表达情况;Western blot法检测CyclinD1、MMP-2、MMP-9蛋白表达情况。结果:与空白对照组比较,100、200、400μmol/L布比卡因组细胞增殖能力和CyclinD1、MMP-2、MMP-9蛋白表达水平逐渐降低,S期细胞比例逐渐降低,迁移及侵袭细胞数逐渐减少,miR-143-3p表达水平和G1期细胞比例逐渐升高(P<0.05)。与miR-NC组比较,miR-143-3p组细胞增殖能力和CyclinD1、MMP-2、MMP-9蛋白表达水平降低,迁移及侵袭细胞数减少,S期细胞比例降低,G1期细胞比例逐渐升高(P<0.05)。与抗miR-NC+400μmol/L布比卡因组比较,抗miR-143-3p+400μmol/L布比卡因组细胞增殖能力和CyclinD1、MMP-2、MMP-9蛋白表达水平升高,迁移及侵袭细胞数增多,S期细胞比例升高,G1期细胞比例降低(P<0.05)。结论:布比卡因可通过调控miR-143-3p抑制肝癌细胞Hep3B增殖、迁移、侵袭,诱导细胞周期阻滞。Aim:To explore the effects of bupivacaine on the proliferation, migration and invasion abilities of liver carcinoma cells Hep3B and its possible mechanism.Methods:Hep3B cells were cultured in vitro,and divided into blank control group and 100 μmol/L bupivacaine group, 200 μmol/L bupivacaine group, 400 μmol/L bupivacaine group;miR-negative control(NC) group, miR-143-3p group;anti-miR-NC+400 μmol/L bupivacaine group, anti-miR-143-3p+400 μmol/L bupivacaine group.MTT assay, flow cytometry and Transwell assay were used to detect cell proliferation, cell cycle, migration and invasion.The expression of miR-143-3p was detected by qRT-PCR.CyclinD1,MMP-2,MMP-9 protein expressions were detected by Western blot.Results:Compared with the blank control group, cell proliferation ability, the expression levels of CyclinD1,MMP-2,MMP-9 protein, the proportion of cells in S phase, and the number of migrating and invading cells in 100,200,and 400 μmol/L bupivacaine groups gradually decreased, and the expression level of miR-143-3p and the proportion of cells in G1 phase gradually increased(P<0.05).Compared with miR-NC group, the cell proliferation ability, CyclinD1,MMP-2,MMP-9 protein levels, the number of migrating and invading cells in miR-143-3p group decreased, the proportion of cells in S phase decreased, and the proportion of cells in G1 phase increased(P<0.05).Compared with anti-miR-NC+400 μmol/L bupivacaine group, in anti-miR-143-3p+400 μmol/L bupivacaine group, cell proliferation ability and CyclinD1,MMP-2,MMP-9 protein levels increased, the number of migrating and invading cells increased, the proportion of cells in S phase increased, and the proportion of cells in G1 phase decreased(P<0.05).Conclusion:Bupivacaine could inhibit the proliferation, migration and invasion, and induce cell cycle arrest of liver carcinoma cells Hep3B by regulating miR-143-3p.

关 键 词：肝癌 HEP3B细胞 布比卡因 miR-143-3p 细胞增殖 迁移 侵袭 

分 类 号：R735.7[医药卫生—肿瘤]