双酚类化合物对人胚肾细胞HEK293的毒性和氧化应激的研究  

Effects of Bisphenols on Cytotoxicity and Oxidative Stress in Human Embryonic Kidney Cells HEK293

作　　者：李专 陈力可 汪雪格 李广柱[1,2] 边德军 LI Zhuan;CHEN Li-ke;WANG Xue-ge;LI Guang-zhu;BIAN De-jun(School of Water Conservancy&Environment Engineering,Changchun Institute of Technology,Changchun 130012,China;Jilin Key Laboratory of Urban Sewage Treatment,Changchun 130012,China;Hainan Academy of Environmental Sciences,Haikou 571126,China)

机构地区：[1]长春工程学院水利与环境工程学院,长春130012 [2]吉林省城市污水处理重点实验室,长春130012 [3]海南省环境科学研究院,海口571126

出　　处：《科学技术与工程》2023年第2期863-869,共7页Science Technology and Engineering

基　　金：吉林省教育厅科学技术研究项目(JJKH20210700KJ);海南省自然科学基金(422RC801);长春市科技计划(21ZGM12)。

摘　　要：双酚类化合物(bisphenols,BPs)是一类新型环境有机污染物,BPs排放到环境介质后经多种途径进入生物体并在体内蓄积,已逐渐成为威胁环境和健康的重要风险因子。为探明BPs对人体健康的潜在毒性效应,选取人胚肾细胞HEK293作为模型,通过测定细胞活力、胞内活性氧含量、氧化应激参数(SOD、GSH、MDA)及细胞内钙离子水平,比较了双酚A(bisphenol A,BPA)、双酚S(bisphenol S,BPS)、双酚F(bisphenol F,BPF)和双酚AF(bisphenol AF,BPAF)对HEK293细胞的毒性影响。结果表明,BPA、BPS、BPF和BPAF能够不同程度的抑制HEK293细胞活力,通过促进氧化应激产生ROS,降低抗氧化能力,迅速提高细胞内Ca2+水平,诱导HEK293细胞损伤。在本文研究所选的浓度范围内,BPAF对HEK293细胞的毒性影响最大,BPA和BPF对HEK293细胞的毒性影响次之,BPS对HEK293细胞的毒性影响较小。研究结果为深化了解双酚类化合物的毒理学系统研究和风险评估提供参考依据。Bisphenols(BPs),a type of emerging environmental organic contaminants,are discharged into the environment and accumulated in animals and human bodies.Bisphenols have become an important risk factor threatening the environment and health.In order to explore the potentially toxic effects of BPs on human health,HEK293 cell was selected as a model,the toxicity of four BPs(BPA,BPS,BPF and BPAF)were systematically compared by measuring survival rate,intracellular reactive oxygen species(ROS)content,oxidative stress parameters(SOD,GSH,MDA)and intracellular Ca2+level.The results show that bisphenols inhibite HEK293 cell viability to varying degrees,induce ROS generation by promoting oxidative stress,reduce antioxidant capacity,increase intracellular calcium ions level,and induce HEK293 cell damage.Within the concentration range selected,BPAF displayes the strongest toxicity effects on HEK293 cells,followes by BPA and BPF,while BPS showes the weakest toxicity effects on HEK293 cells.The results presente will provide basic information for evaluating the toxic effects and risk assessment of bisphenols.

关 键 词：双酚类化合物 人胚肾细胞 氧化应激 钙离子 

分 类 号：X174[环境科学与工程—环境科学]