ACE2在鼻咽癌中的表达及对鼻咽癌细胞增殖的影响  

Expression of ACE2 in nasopharyngeal carcinoma and the related effect on the proliferation of cancer cells

作　　者：万仁强[1] 刘敏婷 翁泽平[2] 裴娜娜 WAN Renqiang;LIU Minting;WENG Zeping;PEI Nana(Department of Otolaryngology Head and Neck Surgery,GuangDong Second Provincial General Hospital,Guangzhou 510317,China;Department of Pathology,the first affiliated Hospital of Jinan University,Guangzhou 510630,China)

机构地区：[1]广东省第二人民医院耳鼻咽喉头颈外科,广东广州510317 [2]暨南大学附属第一医院病理科,广东广州510630

出　　处：《暨南大学学报（自然科学与医学版）》2022年第6期573-580,共8页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：国家自然科学基金青年科学基金项目(81702688);广东省医学科学技术研究基金项目(A2019506);广州市基础研究计划基金项目(202102021105);广东省第二人民医院青年基金项目(YQ2017-004)。

摘　　要：目的:探讨血管紧张素转化酶2(ACE2)在鼻咽癌中的表达并观察ACE2激动剂二乙酰胺三氮脒(DIZE)对鼻咽癌细胞增殖的影响,以及对ACE2、血管紧张素Ⅱ(AngⅡ)、血管紧张素(1-7)[Ang(1-7)]和表皮生长因子受体(EGFR)表达的影响。方法:采用免疫组织化学(IHC)En Vision法检测ACE2在66例鼻咽癌、175例鼻咽黏膜慢性炎组织中的表达,以及EGFR在88例鼻咽癌组织中的表达;采用荧光定量RT-PCR法检测ACE2在鼻咽癌细胞中的表达;处理鼻咽癌细胞,采用MTT及平板克隆形成实验检测其对鼻咽癌细胞生长的影响,检测DIZE对ACE2、AngⅡ、Ang(1-7)和EGFR表达的影响。结果:在66例鼻咽癌标本中,均未观察到ACE2的表达,而在黏膜慢性炎标本中,ACE2表达的阳性率为69.7%;鼻咽癌细胞中ACE2的表达显著降低,且与细胞的分化相关;在鼻咽癌组织中EGFR高表达,阳性率为97.7%;DIZE作用于鼻咽癌细胞后,ACE2及Ang-(1-7)的表达升高,AngⅡ与EGFR的表达降低,并且可抑制鼻咽癌细胞的生长及克隆形成。结论:ACE2在鼻咽癌组织与细胞中低表达,ACE2激动剂DIZE可提高ACE2的水平,并且可抑制鼻咽癌细胞增殖。Objective:To investigate the expression of angiotensin converting enzyme 2(ACE2)in nasopharyngeal carcinoma(NPC),and the effect of ACE2 agonist,diminazene aceturate(DIZE),on proliferation of NPC cells,the expression of ACE2,angiotensinⅡ(ANGⅡ),angiotensin(1-7)[ang(1-7)]and epidermal growth factor receptor(EGFR).Methods:Immunohistochemistry(IHC)was conducted to detect the expression of ACE2 in 66 NPC tissue specimens,in 175 nasopharyngitis tissues,and EGFR expression in 88 NPC tissues.The expression of ACE2 in NPC cells were detected by qPCR.MTT and colony formation assay were performed to determine the effect of DIZE on the proliferation of NPC cells.qPCR or enzyme linked immunosorbent assay(ELISA)were used to determine the AngⅡ,ang(1-7)and EGFR levels of NPC cells treated with DIZE.Results:In the present study,the expression of ACE2 was not detected in 66 NPC tissues,while ACE2 was increased in control samples,and the positive rate reached up to 69.7%.The ACE2 expression was also decreased in NPC cells,which correlated with cancer cells’differentiation.EGFR was overexpressed in NPC specimens,and the positive rate was about 97.7%in our study.Treatment the NPC cells with DIZE,resulting in a decrease in cell growth,cloney formation and the expression of AngⅡand EGFR,while the expression of ACE2 and Ang-(1-7)was increased.Conclusion:ACE2 was decreased in NPC tissues and cells,and ACE2 agonist DIZE increased the level of ACE2 and inhibit the proliferation of NPC cells.

关 键 词：血管紧张素转化酶2 表皮生长因子受体 细胞增殖 鼻咽癌 二乙酰胺三氮眯 

分 类 号：R739.6[医药卫生—肿瘤]