响应面法优化活性炭对附子多糖的脱色工艺及抗氧化性研究  

Decolorization Process Optimization of Aconiti Lateralis Radix Praeparata Polysaccharide with Activated Carbon by Response Surface Methodology and its Antioxidant Activity

作　　者：杨申明[1] 陈靖[1] 宁娜 王振吉[1] YANG Shenming;CHEN Jing;NING Na;WANG Zhengji(Department of Resources,Environment and Chemistry,Chuxiong Normal University,Chuxiong 675000,China)

机构地区：[1]楚雄师范学院资源环境与化学学院,云南楚雄675000

出　　处：《化工技术与开发》2023年第1期18-22,共5页Technology & Development of Chemical Industry

基　　金：云南省中青年学术和技术带头人后备人才项目(2019HB055);云南省“高层次人才培养支持计划”青年拔尖人才项目(YNWR-QNBJ-2020-104);“彝乡科技领军人才”项目。

摘　　要：采用响应面分析法优化活性炭对附子多糖的脱色工艺,并测定了脱色后的附子多糖的抗氧化性。根据前期的单因素实验结果,以活性炭的用量、脱色的温度和脱色的时间为自变量,脱色率为响应值,利用Box-Benhnken中心组合实验并结合响应面分析法,优化附子多糖的脱色工艺,并通过测定DPPH自由基和羟基自由基的清除率,来评价脱色后的附子多糖的抗氧化性。结果表明,附子多糖的最佳脱色工艺条件为:活性炭用量为1.4%(m/m),脱色温度60℃,脱色时间52min,在此条件下,附子多糖的平均脱色率为84.99%,与预测值(85.22%)接近;附子多糖的质量浓度为0.018~0.090 mg·mL^(-1)时,对DPPH·和·OH的清除率,最大分别为85.54%和72.64%。In this paper,response surface methodology was used to optimize the decolorization process of polysaccharides from Aconiti Lateralis Radix Praeparata with activated carbon,and the antioxidant activity of polysaccharides after decolorization was determined.On the basis of previous single factor experiments,the active carbon dosage,decolorization temperature and decolorization time were taken as independent variables,and the decolorization rate was the response value.The decolorization technology of Aconiti Lateralis Radix Praeparata polysaccharide was optimized by Box-Benhnken center combination test and response surface analysis.The antioxidant activity of polysaccharides after decolorization was evaluated by determining the scavenging rate of DPPH radicals and hydroxyl radicals.The results showed that the optimum decolorization conditions were as followed:the amount of activated carbon was 1.4%(m/m),the decolorization temperature was 60℃and the decolorization time was 52min.Under these conditions,the average decolorization rate was 84.99%,which was closed to the predicted value(85.22%).When the mass concentration of Aconiti Lateralis Radix Praeparata polysaccharide was 0.018~0.090 mg/mL,the maximum clearance rates of DPPH·and·OH were 85.54%and 72.64%respectively.

关 键 词：附子 多糖 响应面法 活性炭 脱色 抗氧化性 

分 类 号：O269.12[理学—数学]