神经生长因子对坐骨神经损伤大鼠脊髓内神经纤维的影响  

The effects of nerve growth factor on nerve fibers within spinal cord in rats with sciatic nerve injury

作　　者：杨林凤 黄静[2] 杨雁琪 陈熙蕾 易泓妤 黄美玲 杨正伟[1] 彭彬[1] Yang Linfeng;Huang Jing;Yang Yanqi;Chen Xilei;Yi Hongyu;Huang Meiling;Yang Zhengwei;Peng bin(Institute of Basic Medicine and Forensic Medicine,North Sichuan Medical College,Nanchong 637000,China;Science and Technology Innovation Center,North Sichuan Medical College,Nanchong 637000,China;Department of Stomatology,North Sichuan Medical College,Nanchong 637000,China;Department of Clinical Medicine,North Sichuan Medical College,Nanchong 637000,China;Department of Medical Laboratory,North Sichuan Medical College,Nanchong 637000,China)

机构地区：[1]川北医学院基础医学与法医学研究所,南充637000 [2]川北医学院科技创新中心,南充637000 [3]川北医学院口腔医学系,南充637000 [4]川北医学院临床医学系,南充637000 [5]川北医学院医学检验系,南充637000

出　　处：《神经解剖学杂志》2022年第6期677-682,共6页Chinese Journal of Neuroanatomy

基　　金：四川省大学生创新创业计划项目(S20211063071,S20211063111,S20211063014);南充市科学技术局博士、硕士学位授权点和“一流学科”建设专项(20SXQT0304)。

摘　　要：目的:确定坐骨神经慢性压迫性损伤(CCI)所致神经病理性痛(NP)是否伴有脊髓背角内有髓或无髓神经纤维长度密度或体积分数的变化,以及神经生长因子(NGF)干预的作用。方法:3月龄雄性SD大鼠随机分为sham组、CCI组和CCI+NGF组,术后28 d取材,将每只大鼠左侧和右侧L5脊髓背角各分成4个组织块并分别制成1张各向同性Epon812环氧树脂包埋超薄切片,利用透射电镜(TEM)在每张超薄切片内等距随机抽选视野拍照后,采用体视学方法计算脊髓背角内有髓神经纤维和无髓神经纤维的体积分数、长度密度以及有髓神经纤维的直径及其髓鞘厚度。结果:与未手术侧比较,三组大鼠手术侧L5节段脊髓背角内有髓神经纤维或无髓神经纤维的体积分数或长度密度以及有髓神经纤维的直径或髓鞘厚度之间的差异均无统计学意义,三组大鼠相同侧别的组间比较其差异也无统计学意义。结论:CCI所致NP以及NGF干预不会引起脊髓背角内有髓神经纤维和无髓神经纤维总体积或总长度的改变。Objective: To determine whether peripheral neuropathic pain(NP) induced by chronic constriction injury(CCI) is accompanied by changes in the length density or volume fraction of myelinated or unmyelinated nerve fibers in the rat spinal dorsal horn and the effects of nerve growth factor(NGF) on it. Methods: Three months old male Sprague-Dawley rats were randomly divided into sham group, CCI group and CCI+NGF group. Twenty-eight days after operation, L5segment of the spinal cord of each animal was removed, and then, the left and right spinal dorsal horn were divided into four tissue blocks respectively, and one isotropic Epon812-embedded ultra-thin section was respectively cut from each tissue block. The testing fields were equally spaced sampled and taken photos from each section with transmission electron microscope(TEM), the volume fraction or length density of the myelinated or unmyelinated fibers and the diameter and myelin sheath thickness of myelinated fibers in spinal dorsal horn were estimated with the stereological methods. Results: Compared with the non-operative side, the volume fraction or length density of myelinated nerve fibers or unmyelinated nerve fibers, and the diameter or myelin sheath thickness of myelinated nerve fibers in the spinal dorsal horn of L5segment on the operative side of the three groups were not statistically significant, and also, there was no significant difference on the same side among three groups. Conclusion: CCI-induced NP and NGF intervention do not lead to significant changes in the total volume or length of myelinated and unmyelinated nerve fibers in the spinal dorsal horn.

关 键 词：神经生长因子 慢性压迫性神经损伤 神经病理性痛 脊髓背角 体视学 神经纤维 大鼠 

分 类 号：R745[医药卫生—神经病学与精神病学]