一种巨大腹主动脉瘤大鼠模型制作方法  

A New Method to Make Rat Model of Giant Abdominal Aortic Aneurysm

作　　者：刘新农 杨根欢 廖鹏志 汪岩[1] 贾玉龙[1] LIU Xinnong;YANG Genhuan;LIAO Pengzhi;WANG Yan;JIA Yulong(Department of Vascular Surgery,Beijing Tiantan Hospital,Capital Medical University,Beijing 100070,China)

机构地区：[1]首都医科大学附属北京天坛医院血管外科,北京市100070

出　　处：《中国分子心脏病学杂志》2022年第6期5065-5072,共8页Molecular Cardiology of China

摘　　要：目的 建立一种巨大腹主动脉瘤(GAAA)大鼠模型的制作方法。方法 以Sprague-Dawley大鼠为研究对象分为3组,分别为改良法腹主动脉腔内灌注猪胰蛋白酶(PPE)组(GAAA组),传统腹主动脉腔内灌注PPE组(传统AAA组)和生理盐水组,每组10只。超声检测腹主动脉直径并计算扩张率,扩张率大于150%视为腹主动脉瘤,大于400%视为GAAA。苏木精-伊红(HE)染色、弹力纤维(EVG)染色和免疫组织化学(IHC)染色鉴定腹主动脉平滑肌细胞、弹力纤维降解和炎症细胞浸润情况。结果大鼠均成功灌注PPE,随访期间无大鼠死亡。术后2周,GAAA组腹主动脉平均扩张率为424.82%±83.66%,其中70%(n=7)大鼠符合GAAA成瘤标准;传统AAA组腹主动脉平均扩张率为221.80%±34.66%,无大鼠符合GAAA成瘤标准。组织学结果示GAAA组内平滑肌细胞数量减少、弹力纤维明显降解和巨噬细胞浸润较传统AAA组更为明显。生理盐水组未出现腹主动脉瘤。结论 改良法腹主动脉腔内灌注PPE是一种制作GAAA大鼠模型的实验方法,该模型成瘤率高,具有与人GAAA相似的病理学特点。Objective To establish a new method to make rat model of giant abdominal aortic aneurysm(GAAA).Methods SD rats were divided into three groups:the modified intra-abdominal aortic infusion of elastase from porcine pancreas(PPE)group(GAAA group),the traditional intra-abdominal aortic infusion of PPE group(traditional AAA group) and normal saline group(n =10 in each group).The diameter of abdominal aorta was detected by ultrasound and the dilatation rate was calculated.The dilatation rate greater than 150% was considered as abdominal aortic aneurysm and the dilatation rate greater than 400%was considered as GAAA.Results All rats perfused with PPE did not die during the follow-up period.At 2 weeks,the mean dilatation rate of rats in GAAA group was 424.82% ± 83.66% and the formation rate of GAAA was 70%(n = 7).The mean dilatation rate of rats in traditional AAA group was 221.80% ± 34.66%,and no rats meet the criterion of GAAA.The histological results showed a decrease in numbers of smooth muscle cells,degradation of elastic fibers and massive macrophages infiltration in GAAA group was more obvious compared with the traditional AAA group.No abdominal aortic aneurysm was observed in normal saline group.Conclusions The modified intra-abdominal aortic infusion of PPE represents a method to induce rat model of GAAA and the pathological features of the present model are similar to those patients with GAAA.

关 键 词：巨大腹主动脉瘤 动物模型 猪胰蛋白酶 大鼠 手术 

分 类 号：R-332[医药卫生] R543.16