超声波辅纤维素酶提取泡桐花总黄酮工艺及其抗氧化研究  

Ultrasound-assisted cellulase of extraction process of Paulownia flower total flavonoids and its antioxidant research

作　　者：朱秀红[1] 韩钰 茹广欣[1] ZHU Xiu-hong;HAN Yu;RU Guang-xin

机构地区：[1]河南农业大学林学院,河南郑州450003

出　　处：《饲料研究》2022年第24期73-78,共6页Feed Research

基　　金：河南省财政科技兴林项目(项目编号:30602126);河南省教学名师工作室(项目编号:21XM0413)。

摘　　要：试验旨在使用超声波辅助纤维素酶提取泡桐花总黄酮,研究泡桐花总黄酮的抗氧化活性。采用Box-Behnken响应面试验设计优化了最佳提取工艺参数,得到拟合较良好的回归方程与构建较理想的回归模型。结果显示:泡桐花总黄酮提取最优提取工艺为:液料比46 mL/g、酶解温度62℃、酶解时间116 min、乙醇体积分数70%。在此工艺条件下,总黄酮提取率达到73.92 mg/g。泡桐花总黄酮对DPPH自由基清除率略低于维生素C,但仍达到了86.22%,具有较强的抗氧化性。研究表明,泡桐花总黄酮可作为天然绿色抗氧化添加剂。The experiment was to use ultrasonic assisted cellulase to extract total flavonoids from Paulownia flower,and study the antioxidant activity of total flavonoids from Paulownia flower.Box-Behnken response surface experiment was used to optimize the optimal extraction process parameters,and a better fitting regression equation was obtained and a better regression model was constructed.The results showed that the optimal extraction conditions were as follows:Liquid-solid ratio 46 mL/g,enzymolysis temperature 62℃,enzymolysis time 116 min,ethanol volume fraction 70%.Under these conditions,the extraction yield of total flavonoids reached 73.92 mg/g.In DPPH free radical scavenging experiment,the free radical scavenging rate of total flavonoids in Paulownia flower was slightly lower than that of VC,but still reached 86.22%,which showed strong antioxidant activity.The study indicates that total flavonoids of Paulowinia flower can be used as natural green antioxidant additives.

关 键 词：泡桐花 总黄酮 抗氧化活性 响应面优化试验 

分 类 号：S816.7[农业科学—饲料科学]