MS相关的hsa-miR-17-5p靶基因预测和关键基因筛选  

MS-associated hsa-miR-17-5p target gene prediction and bioinformatics analysis

作　　者：冀彩丽 桑道乾[2] 宋传旺 JI Cai-li(Department of Immunology,College of Laboratory Medicine,Bengbu Medical College,Bengbu 233030,China)

机构地区：[1]蚌埠医学院检验医学院免疫学教研室,安徽蚌埠233004 [2]蚌埠医学院第一附属医院神经内科,安徽蚌埠233004

出　　处：《牡丹江医学院学报》2023年第1期38-43,共6页Journal of Mudanjiang Medical University

基　　金：蚌埠医学院“512人才培育计划”项目(by51201103);蚌埠医学院科研创新团队项目(BYKC201902)。

摘　　要：目的对人类miR-17-5p(hsa-miR-17-5p)靶基因预测和富集分析,并结合基因的差异表达寻找多发性硬化症的生物学标志物。方法利用生物信息学方法,预测hsa-miR-17-5p靶基因,对预测结果进行基因本体数据库(Gene Ontology,GO)、京都基因和基因组百科全书(Kyoto Encyclopedia of Genes and Genome,KEGG)富集分析,并构建蛋白质互作网络图(Protein-Protein Interaction Networks,PPI)。再结合多发性硬化症患者和健康对照组的差异表达基因,进一步筛选出hsa-miR-17-5p对多发性硬化症影响的关键基因。最后,利用RNA22 v2验证关键基因是否存在结合位点。结果不同网站预测后,取交集获取376个公共靶基因,这些靶基因有复杂的相互作用,主要存在于胞浆与核质,参与多种转录调控和蛋白质磷酸化等生物学过程,同时也与癌症、病毒感染和免疫系统等信号通路相关;靶基因和多发性硬化症患者的相对下调基因有三个共同基因PDLIM5、KCNB1、BTBD7。进一步验证上述关键基因结合位点,发现仅有PDLIM5存在P值小于0.05的结合位点。结论hsa-miR-17-5p靶基因参与多种生物学过程,而且PDLIM5、KCNB1、BTBD7基因很可能是hsa-miR-17-5p对多发性硬化症产生影响的重要桥梁,其中PDLIM5尤为重要。Objective To predict and enrich the analysis of human miR-17-5p(hsa-miR-17-5p)target genes and to search for biological markers for multiple sclerosis(MS)in combination with differential expression of the genes.Methods Using a bioinformatics approach,hsa-miR-17-5p target genes were predicted.The predicted results were analyzed by Gene Ontology(GO),Kyoto Encyclopedia of Genes and Genome(KEGG)enrichment,and the Protein-Protein Interaction Networks(PPI)maps were constructed.The differentially expressed genes of MG patients and healthy controls were combined to further screen the key genes for the effect of hsa-miR-17-5p on MG.Finally,RNA22 v2 was used to verify the existence of binding sites for key genes.Results Different software were used to predict the target genes and then the intersection was took to obtain 376 genes.These target genes had complex interactions,mainly existed in the cytoplasm and nucleoplasm,and were involved in a variety of biological processes such as transcriptional regulation and protein phosphorylation,and were also associated with signaling pathways such as cancer,viral infection,and the immune system.There were three common genes PDLIM5,KCNB1,and BTBD7 that were relatively down-regulated by these target genes and MS patients.Further verification of the above key gene binding sites revealed that only PDLIM5 had a binding site with P value less than 0.05.Conclusion Hsa-miR-17-5p target genes are involved in a variety of biological processes,and PDLIM5,KCNB1 and BTBD7 genes are likely to be an important bridge for hsa-miR-17-5p to affect multiple sclerosis,among which PDLIM5 is particularly important.

关 键 词：多发性硬化症 hsa-miR-17-5p 生物信息学 基因 

分 类 号：R744.51[医药卫生—神经病学与精神病学]