硫利达嗪对胰腺癌细胞增殖及凋亡的影响及机制探讨  

The effect of Thioridazine on proliferation and apoptosis of pancreatic cancer cells

作　　者：罗娜 倪猛[2] 刁云辉[2] LUO Na;NI Meng;DIAO Yunhui(Department of Western Medicine,Nanyang Central Hospital,Nanyang 473000,China;Department of Gastroenterology,Nanyang Central Hospital,Nanyang 473000,China)

机构地区：[1]南阳市中心医院西药药学部,473000 [2]南阳市中心医院消化内科,473000

出　　处：《天津医药》2023年第3期230-234,共5页Tianjin Medical Journal

基　　金：南阳市科技攻关项目(192102310326)。

摘　　要：目的探讨硫利达嗪(TDZ)对胰腺癌细胞SW1990增殖、凋亡的影响及其对LINC00470的调控作用。方法用不同浓度(5、10、20μmol/L)的TDZ处理人胰腺癌细胞SW1990,si-NC(si-NC组)、si-LINC00470(siLINC00470组)转染至SW1990细胞,分别将pcDNA(TDZ+pcDNA组)、pcDNA-LINC00470(TDZ+pcDNA-LINC00470组)转染至SW1990细胞后加入TDZ处理细胞;MTT、平板克隆形成实验检测细胞增殖及克隆形成能力;流式细胞术检测细胞凋亡率;实时荧光定量PCR检测LINC00470的表达量;Western blot检测活化的胱天蛋白酶(cleavedcaspase)3、cleaved-caspase9蛋白表达。结果与对照组比较,TDZ不同剂量组SW1990细胞增殖抑制率、凋亡率和cleaved-caspase3、cleaved-caspase9蛋白水平升高,细胞克隆形成数减少,LINC00470的表达量降低(P<0.05)。与si-NC组比较,si-LINC00470组SW1990细胞增殖抑制率、凋亡率和cleaved-caspase3、cleaved-caspase9蛋白水平升高,细胞克隆形成减少(P<0.05)。转染pcDNA-LINC00470可逆转TDZ对SW1990细胞增殖、凋亡及克隆形成的作用。结论TDZ可通过抑制LINC00470的表达而抑制胰腺癌细胞增殖、克隆形成及诱导细胞凋亡。Objective To explore the effect of thioridazine(TDZ)on proliferation and apoptosis of pancreatic cancer cells SW1990 and its regulatory effect on LINC00470.Methods Different concentrations of TDZ(5,10 and 20μmol/L)were used to treat human pancreatic cancer cells SW1990.si-NC and si-LINC00470 were transfected into SW1990 cells(the si-NC group and the si-LINC00470 group).After transfecting pcDNA and pcDNA-LINC00470 into SW1990 cells,TDZ were added to treat cells(the TDZ+pcDNA group and the TDZ+pcDNA-LINC00470 group).MTT,plate clone formation test were used to detect cell proliferation and clone formation ability.Flow cytometry was used to detect the apoptosis rate.The real-time fluorescent quantitative PCR was used to detect the expression level of LINC00470.Western blot assay was used to detect expression levels of activated aspartate specific cysteine protease(cleaved-caspase)3 and cleaved-caspase9.Results Compared with the control group,the cell proliferation inhibition rate,apoptosis rate,cleaved-caspase3 and cleaved-caspase9 protein levels of SW1990 cells were increased,the number of cell clone formation was decreased,and the expression level of LINC00470 was decreased in the different dose TDZ groups(P<0.05).Compared with the si-NC group,the cell proliferation inhibition rate,apoptosis rate,cleaved-caspase3 and cleaved-caspase9 protein levels of SW1990 cells were increased in the si-LINC00470 group,and the cell clone formation was reduced(P<0.05).The transfection of pcDNALINC00470 could reverse the effect of TDZ on cell proliferation,apoptosis and colony formation of SW1990 cells.Conclusion TDZ can inhibit the proliferation,clone formation and induce apoptosis of pancreatic cancer cells by inhibiting the expression of LINC00470.

关 键 词：胰腺肿瘤 硫利达嗪 细胞增殖 细胞凋亡 RNA 长链非编码 LINC00470 

分 类 号：R735.9[医药卫生—肿瘤] R979.1[医药卫生—临床医学]