参芪注射液协同IFN-α抑制肝癌细胞体外增殖、迁移能力和侵袭性的研究  

Shenqi Fuzheng injection combined with interferon alpha on the proliferation and invasiveness of hepatocellular carcinoma cells in vitro

作　　者：刘美君 丁治国[2] 陈晓珩[1] 祁烁[2] 李会龙[1] 户蕊 李璐[1] 商建伟[1] 李哲[1] LIU Mei-jun;DING Zhi-guo;CHEN Xiao-heng;QI Shuo;LI Hui-long;HU Rui;LI Lu;SHANG Jian-wei;LI Zhe(Department of Thyroid Diseases,Dongzhimen Hospital of Beijing University of Chinese Medicine,Beijing 100700,China;Department of Thyroid Diseases,SUN Si Miao Hospital of Beijing University of Chinese Medicine,Tongchuan 727031,China)

机构地区：[1]北京中医药大学东直门医院甲状腺病科,北京100700 [2]北京中医药大学孙思邈医院甲状腺病科,陕西铜川727031

出　　处：《中国现代普通外科进展》2022年第12期925-927,932,共4页Chinese Journal of Current Advances in General Surgery

基　　金：国家自然科学基金项目(81673972)。

摘　　要：目的:探讨参芪扶正注射液协同IFN-α对肝癌SMMC-7721细胞体外增殖与侵袭力的影响。方法:在SMMC-7721体外培养系中加入小剂量参芪扶正注射液(0.5 g/L)及IFN-α,MTT法检测参芪扶正注射液协同IFN-α对SMMC-7721细胞增殖作用的影响;Transwell检测参芪扶正注射液对SMMC-7721细胞迁移能力的抑制作用;Invasion实验检测参芪扶正注射液对SM M C-7721细胞增殖能力的影响。结果:与单独使用IFN-α相比较,参芪扶正注射液联合IFN-α能够降低SMMC-7721细胞的增殖能力(P<0.05);同时还能够抑制SMMC-7721细胞的迁移能力(P<0.05)以及侵袭能力(P<0.05)。结论:参芪扶正注射液协同IFN-α在体外可以有效抑制SMMC-7721细胞的增殖能力,并降低其迁移力及侵袭性。Objective: To determine the synergistic effect of Shenqi Fuzheng injection combined with interferon alpha on the proliferation and invasiveness of hepatocellular carcinoma SMMC-7721 cells in vitro.Methods:After adding Shenqi Fuzheng injection(0.5 g/L) and interferon alpha(IFN-α) to the SM MC-7721 in vitro culture system for 24 hours,the effect of Shenqi Fuzheng injection and interferon alpha on the proliferation of SMMC-7721 cells was detected by MTT method.The effect of Shenqi Fuzheng injection on the migration ability of SMMC-7721 cells was detected by Transwell;the effect of Shenqi Fuzheng injection on the proliferation ability of SMMC-7721 cells was detected by Invasion test.Results:Compared with the use of interferon alpha alone,Shenqi Fuzheng injection combined with interferon alpha could reduce the proliferation ability of SMMC-7721 cells(P<0.05);at the same time,it could also inhibit the migration ability of SMMC-7721 cells(P<0.05).and invasive ability(P<0.05).Conclusion:Shenqi Fuzheng injection combined with interferon alpha can effectively inhibit the proliferation of SMMC-7721 cells in vitro,and reduce their migration and invasiveness.

关 键 词：肝肿瘤 参芪扶正注射液 增殖 迁移能力 侵袭力 

分 类 号：R735.7[医药卫生—肿瘤]