lncRNA CERS6-AS1通过调控miR-138-2-3p抑制人胶质瘤细胞系增殖  

lncRNA CERS6-AS1 Inhibits the Proliferation of Human Glioma Cell Lines by Regulating miR-138-2-3p

作　　者：黄琦[1] 翟海程[1] HUANG Qi;ZHAI Haicheng(Department of Neurosurgery,Hanzhong Central Hospital,Hanzhong,Shaanxi,723000,China)

机构地区：[1]汉中市中心医院神经外科,陕西省汉中市723000

出　　处：《医学分子生物学杂志》2023年第1期20-25,共6页Journal of Medical Molecular Biology

基　　金：汉中市中心医院科研项目(No.YK1807)。

摘　　要：目的探讨lncRNA CERS6-AS1对胶质瘤细胞生物学行为的影响及其可能作用机制.方法qRT-PCR法检测胶质瘤组织、癌旁组织中CERS6-AS1和miR-138-2-3p的表达量;Pearson法分析胶质瘤组织中CERS6-AS1与miR-138-2-3p表达量的相关性;体外培养人胶质瘤细胞T98G,将si-NC、si-CERS6-AS1、miR-NC、miR-138-2-3p mimics、si-CERS6-AS1与anti-miR-NC、si-CERS6-AS1与anti-miR-138-2-3p分别转染至T98G细胞;CCK-8法、平板克隆形成实验、Transwell小室实验分别检测细胞增殖、克隆形成、迁移及侵袭能力;双荧光素酶报告基因实验检测CERS6-AS1和miR-138-2-3p的靶向关系.结果与癌旁组织比较,胶质瘤组织中CERS6-AS1的表达量升高(P<0.05),miR-138-2-3p的表达量降低(P<0.05);CERS6-AS1与miR-138-2-3p呈负相关(r=-0.8899,P<0.001);si-CERS6-AS1组细胞活力、克隆形成细胞数、迁移及侵袭细胞数均低于si-NC组(P<0.05);CERS6-AS1可靶向调节miR-138-2-3p的表达;miR-138-2-3p组细胞活力、克隆形成细胞数、迁移及侵袭细胞数均少于miR-NC组(P<0.05);si-CERS6-AS1+anti-miR-138-2-3p组细胞活力、克隆形成细胞数、迁移及侵袭细胞数均比si-CERS6-AS1+anti-miR-NC组增多(P<0.05).结论干扰CERS6-AS1表达可通过调控miR-138-2-3p而抑制胶质瘤细胞增殖、克隆形成、迁移及侵袭.ObjectiveTo explore the effect of lncRNA CERS6-AS1 on the biological behavior of glioma cells and its possible mechanism.Methods qRT-PCR method was used to detect the expression levels of CERS6-AS1 and miR-138-2-3p in glioma tissues and adjacent tissues.Pearson method was used to analyze the correlation between CERS6-AS1 and miR-138-2-3p expression in glioma tissues.Human glioma cells T98G were cultured in vitro,and then were transfected with si-NC,si-CERS6-AS1,miR-NC,miR-138-2-3p mimics,si-CERS6-AS1 and anti-miR-NC,si-CERS6-AS1 and anti-miR-138-2-3p.The CCK-8 method,plate colony formation experiment,and transwell assay were used to detect the cell capabilities of proliferation,clone formation,migration and invasion.The dual luciferase reporter gene experiment was used to verify the targeting relationship between CERS6-AS1 and miR-138-2-3p.ResultsCompared with the adjacent tissues,the expression level ofCERS6-AS1 in glioma tissues was increased(P<0.05),while the expression level of miR-138-2-3p was decreased(P<0.05).CERS6-AS1 was negatively correlated with miR-138-2-3p(r=-0.8899,P<0.001).CERS6-AS1 could target the expression of miR-138-2-3p.The cell viability,the number of colony forming cells,the number of migration and invasion cells in the si-CERS6-AS1 group were lower than those in the si-NC group(P<0.05).The cell viability,the number of colony formed cells,the number of migrated and invasive cells in the miR-138-2-3p group were less than those in the miR-NC group(P<0.05).The cell viability,the number of clone formed cells,the number of migrated and invasive cells in the si-CERS6-AS1+anti-miR-138-2-3p group were all higher than those in the si-CERS6-AS1+anti-miR-NC group(P<0.05).ConclusionInterference of the expression of CERS6-AS1 could inhibit the proliferation,clone formation,migration and invasion of glioma cells by regulating miR-138-2-3p.

关 键 词：lncRNA CERS6-AS1 miR-138-2-3p 胶质瘤 细胞增殖 侵袭 

分 类 号：R739.41[医药卫生—肿瘤]