Topical Calendula officinalis L.inhibits inflammatory pain through antioxidant,anti-inflammatory and peripheral opioid mechanisms  

作　　者：Bárbara B.Garrido-Suárez Gabino Garrido Addis Bellma Menéndez Nelson Merino Odalys Valdés Nilia de la Paz Aylema Romero Livan Delgado Miguel D.Fernández Octavio Piñeros RenéDelgado-Hernández 

机构地区：[1]Pharmacology and Toxicology,Chemical Analytic and Pharmaceutical Technology Laboratories,Center for Pharmaceutical Research and Development(CIDEM),Havana 10600,Cuba [2]Department of Pharmacology,Institute of Marine Science(ICIMAR),Havana 10300,Cuba [3]Department of Pharmaceutical Sciences,Faculty of Sciences,Catholic University of the North,Antofagasta 1240000,Chile [4]Research Department,University of Santiago de Cali,Cali 760032,Colombia [5]Center for Research and Biological Evaluations,Institute of Pharmacy and Food,University of Havana,Havana 17100,Cuba

出　　处：《Journal of Integrative Medicine》2023年第1期34-46,共13页结合医学学报（英文版）

基　　金：This research was financed by the projects of Ministerio de Salud Pública(MINSAP)Cuba(No.0808001);Agencia Presidencial de Cooperación Internacional de Colombia(APC),Bilateral Program Cuba-Colombia,and Fondo Nacional de Desarrollo Científico y Tecnológico(FONDECYT)Chile(No.1130601).

摘　　要：Objective The present study evaluated the antinociceptive activity of Calendula officinalis L.(Ca)cream on inflammatory hypernociception.Methods Creams with different Ca concentrations were tested for their ability to ameliorate pain-related behavior and edema in rats using formalin test,carrageenan(Cg)-induced acute inflammation model,bradykinin(BK)-induced acute inflammation model,and complete Freund’s adjuvant(CFA)-induced chronic inflammation model.Using the formalin test,we also examined the implication of peripheral opioid receptors in the antinociceptive mechanisms of Ca cream,by means of Q-naloxone,a peripherally acting nonselective opioid antagonist.Furthermore,the effects of Ca cream compared with diclofenac on BK-induced edema were examined when the kininase II in tissue was preserved or inhibited by captopril.The local production of redox biomarkers in formalin model,tumor necrosis factor-α(TNF-α)in Cg model and histopathological changes in CFA model were also evaluated.Results A single application of Ca cream at a dose of 10%or 30%(w/w)decreased the formalin-induced licking/biting behavior during both phases of this test in a Q-naloxone-sensitive manner.This effect was associated with the reduction of oxidative stress in the injured paw and the edema associated with the later phase of formalin-induced pain.A single application of Ca cream(10%or 30%)reduced paw edema and thermal hypernociception in Cg-induced acute inflammation,corresponding with a local decrease in TNF-α.Ca cream also inhibited BK-induced spontaneous nociceptive behavior and paw inflammation in both paradigms studied.Repeated applications of Ca cream also decreased CFA-induced chronic inflammation,mechanical hypernociception and histopathological changes in the paw.Conclusion These results reveal the topical antinociceptive and antiedematogenic effects of Ca cream.A modulatory action on peripheral opioid receptors associated with its antioxidant mechanism may be involved,at least in part,in its analgesic effects.These findings m

关 键 词：Calendulaofficinalis L. Formalintest PAIN HYPERALGESIA Inflammation 

分 类 号：R285.5[医药卫生—中药学]