Effects of electroacupuncture on rats with cognitive impairment:An iTRAQ-based proteomics analysis  

作　　者：Zhe-yan Sa Jin-sen Xu Xiao-hua Pan Shu-xia Zheng Qian-ru Huang Long Wan Xiao-xiang Zhu Cai-lian Lan Xiao-ran Ye 

机构地区：[1]Department of Meridian Research,Fujian Academy of Chinese Medical Sciences,Fuzhou 350003,Fujian Province,China [2]Key Laboratory of Propagated Sensation along Meridian of Fujian Province,Fuzhou 350003,Fujian Province,China

出　　处：《Journal of Integrative Medicine》2023年第1期89-98,共10页结合医学学报（英文版）

基　　金：This work was supported by the Foundation of Fujian Collaborative Innovation Center for Rehabilitation Technology(X2014008-collaboration);Scientific Research Project for Scientific Research Institutions of Provincial Public Interests of Science and Technology Department of Fujian Province(No.2015R1035-14,2016R1033-10,2018R1035-5)。

摘　　要：Objective The study explores the effects of electroacupuncture(EA)at the governing vessel(GV)on proteomic changes in the hippocampus of rats with cognitive impairment.Methods Healthy male rats were randomly divided into 3 groups:sham,model and EA.Cognitive impairment was induced by left middle cerebral artery occlusion in the model and EA groups.Rats in the EA group were treated with EA at Shenting(GV24)and Baihui(GV20)for 7 d.Neurological deficit was scored using the Longa scale,the learning and memory ability was detected using the Morris water maze(MWM)test,and the proteomic profiling in the hippocampus was analyzed using protein-labeling technology based on the isobaric tag for relative and absolute quantitation(iTRAQ).The Western blot(WB)analysis was used to detect the proteins and validate the results of iTRAQ.Results Compared with the model group,the neurological deficit score was significantly reduced,and the escape latency in the MWM test was significantly shortened,while the number of platform crossings increased in the EA group.A total of 2872 proteins were identified by iTRAQ.Differentially expressed proteins(DEPs)were identified between different groups:92 proteins were upregulated and 103 were downregulated in the model group compared with the sham group,while 142 proteins were upregulated and 126 were downregulated in the EA group compared with the model group.Most of the DEPs were involved in oxidative phosphorylation,glycolipid metabolism and synaptic transmission.Furthermore,we also verified 4 DEPs using WB technology.Although the WB results were not exactly the same as the iTRAQ results,the expression trends of the DEPs were consistent.The upregulation of heat-shock proteinβ1(Hspb1)was the highest in the EA group compared to the model group.Conclusion EA can effect proteomic changes in the hippocampus of rats with cognitive impairment.Hspb1 may be involved in the molecular mechanism by which acupuncture improves cognitive impairment.

关 键 词：Cerebral ischemia Vascular cognitive impairment ELECTROACUPUNCTURE PROTEOMICS Isobaric tag for relative Absolute quantitation 

分 类 号：R245.97[医药卫生—针灸推拿学]