Evolutionary targets of gene expression divergence in a complex of closely related pine species  

作　　者：Julia Zaborowska Annika Perry Stephen Cavers Witold MWachowiak 

机构地区：[1]Institute of Environmental Biology,Adam Mickiewicz University in Poznan,Uniwersytetu Poznanskiego 6,Poznan 61-614,Poland [2]UK Centre for Ecology and Hydrology,Edinburgh Site,Bush Estate,Penicuik EH260QB,UK

出　　处：《Journal of Systematics and Evolution》2023年第1期198-212,共15页植物分类学报（英文版）

基　　金：the Polish National Science Centre(UMO 2017/27/B/NZ9/00159),which supported analyses of the expression data and publication of the study;the Natural Environment Research Council(NE/K012177/1)for financing data acquisition.

摘　　要：The environment is a powerful selective pressure for sessile organisms,such as plants,and adaptation to the environment is particularly important for long-lived species,like trees.Despite the importance of adaptive trait variation to the survival and success of trees,the molecular basis of adaptation is still poorly understood.Gene expression patterns in three closely related,but phenotypically and ecologically divergent,pine species were analyzed to detect differentiation that may be associated with their adaptation to distinct environments.Total RNA of Pinus mugo,Pinus uncinata,and Pinus sylvestris samples grown under common garden conditions was used for de novo transcriptome assembly,providing a new reference dataset that includes species from the taxonomically challenging P.mugo complex.Gene expression profiles were found to be very similar with only 121 genes significantly diverged in any of the pairwise species comparisons.Functional annotation of these genes revealed major categories of distinctly expressed transcripts,including wood trait properties,oxidative stress response,and response to abiotic factors such as salinity,drought,and temperature.We discuss putative associations between gene expression profiles and adaptation to different environments,for example,the upregulation of genes involved in lignin biosynthesis in the species,which have adapted to mountainous regions characterized by strong winds and thick snow cover.Our study provides valid candidates for verification of the importance of the gene expression role,in addition to evidence for selection within genomic regions,in the process of ecological divergence and adaptation to higher altitudes in pine taxa.

关 键 词：differential gene expression high-altitude adaptations mountain pines Pinus mugo complex transcriptome assembly 

分 类 号：R73[医药卫生—肿瘤]