PLGA导管复合骨髓间充质干细胞与细胞外基质凝胶移植修复大鼠坐骨神经缺损的生物力学分析  

Biomechanical Analysis of Plga Catheter Combined With Bmscs and Extracellular Matrix Gel Transplantation for Repairing Sciatic Nerve Defects in Rats

作　　者：王怀程 李鹏[2] 汪标文 杜纯宇 于涛[1] Wang Huaicheng;Li Peng;Wang Biaowen;Du Chunyu;Yu Tao(Department of Orthopaedics,China-Japan Union Hospital of Jilin University,Changchun 130031,China;Department of Engineering Mechanics,College of Mechanical Engineering and Aviation,Jilin University,Changchun 130022,China)

机构地区：[1]吉林大学中日联谊医院骨关节科,吉林长春130031 [2]吉林大学机械工程与航空学院工程力学系,吉林长春130022

出　　处：《实用骨科杂志》2023年第2期132-136,共5页Journal of Practical Orthopaedics

摘　　要：目的 研究坐骨神经损伤动物模型以聚乳酸-羟基乙酸共聚物[poly(lactic-co-glycolic acid),PLGA]导管复合骨髓间充质干细胞(bone mesenchymal stem cells, BMSCs)与细胞外基质(extracellular matrix, ECM)凝胶移植后坐骨神经的生物力学特性,为临床提供生物力学等基础研究支持。方法 大鼠坐骨神经损伤模型,分别以自体神经、PLGA导管复合BMSCs、PLGA导管复合BMSCs与ECM凝胶移植6、12、18、24周,对各组大鼠进行大体行为学观察,各组大鼠移植168 d时进行坐骨神经功能指数测量,之后取各组大鼠坐骨神经进行拉伸力学性能实验。结果 PLGA导管复合BMSCs移植组、PLGA导管复合BMSCs与ECM凝胶移植组,在拉伸弹性限度载荷、弹性限应力、最大载荷、最大应力、最大应变、弹性限度应变均大于自体神经移植组,差异有统计学意义(P<0.05);PLGA导管复合BMSCs与ECM凝胶移植组的拉伸弹性限度载荷、弹性限应力、最大载荷、最大应力、弹性限度应变大于PLGA导管复合BMSCs移植组,差异有统计学意义(P<0.05)。结论 PLGA导管复合BMSCs、PLGA导管复合BMSCs与ECM凝胶移植坐骨神经损伤具有明显的修复效果。Objective To study the animal model of sciatic nerve injury by combining poly[(lactic-co-glycolic acid),PLGA]catheters with bone marrow mesenchymal stem cells(bone mesenchymal stem cells,BMSCs)combined with extracellular matrix(extracellular matrix,ECM),biomechanical properties of the sciatic nerve after gel transplantation and provide basic research support for clinical application.Methods The rat sciatic nerve injury model was transplanted with autologous nerve,PLGA catheter combined with BMSCs,PLGA catheter combined with BMSCs and ECM gel for6,12,18,and 24weeks,and the general behavior of rats in each group was observed.The functional index of the sciatic nerve was measured 168days after the transplantation in the rats,and then the sciatic nerves of the rats in each group were taken for tensile mechanical properties experiments.Results The tensile elastic limit load,elastic limit stress,maximum load,maximum stress,maximum strain,and elastic limit strain of the PLGA catheter combined with BMSCs transplantation group and the PLGA catheter combined with BMSCs and ECM gel transplantation group were greater than those of the autologous nerve transplantation group.The difference was statistically significant(P <0.05).The tensile elastic limit load,elastic limit stress,maximum load,maximum stress,and elastic limit strain of the PLGA catheter composite BMSCs and ECM gel transplantation group were greater than those of the PLGA catheter composite BMSCs transplantation group.The difference was statistically significant(P <0.05)Conclusion PLGA catheter combined with BMSCs,PLGA catheter combined with BMSCs and ECM gel transplantation have obvious repair effect on sciatic nerve injury.

关 键 词：动物模型 聚乳酸-羟基乙酸共聚物导管 骨髓间充质干细胞 坐骨神经 损伤 

分 类 号：R745[医药卫生—神经病学与精神病学]