miR-219-5p靶向SOX5在口腔癌中的作用初探  

Possible Roles of MiR-219-5p Targeting SOX5 in the Development of Oral Cancer

作　　者：周婷[1] 何影[1,2] 董晓函 习杨彦彬 陈波 佟钧[5] 毛瑞 ZHOU Ting;HE Ying;DONG Xiaohan;XIYANG Yanbin;CHEN Bo;TONG Jun;MAO Rui(School of Stomatology,Kunming Medical University,Kunming Yunnan 650500;Outpatient Department,Yunnan Province Corps Hospital,Chinese People’s Armed Police Forces,Kunming Yunnan 650100;Institute of Neuroscience,Basic Medical College;Science and Technology Achievement Incubation Center;Physical Education Department,Kunming Medical University,Kunming Yunnan 650500,China)

机构地区：[1]昆明医科大学口腔医学院,云南昆明650500 [2]武警云南省总队医院门诊部,云南昆明650100 [3]昆明医科大学基础医学院,云南昆明650500 [4]昆明医科大学科技成果孵化中心,云南昆明650500 [5]昆明医科大学体育部,云南昆明650500

出　　处：《昆明医科大学学报》2023年第2期45-51,共7页Journal of Kunming Medical University

基　　金：云南省教育厅科学研究基金项目(2019J1292)。

摘　　要：目的 研究miR-219-5p与SOX5在口腔鳞状细胞癌中是否存在差异性表达,并探讨miR-219-5p对SOX5的靶向调控作用,方法 运用口腔鳞状细胞癌细胞株SCC4及SCC9,人正常皮肤成纤维细胞系(HF)作为对照,对miR-219-5p与SOX5在个细胞系中的表达情况进行检测(RT-qPCR、Western blot);并在SCC4及SCC9细胞株中验证miR-219-5p对SOX5表达的靶向调控作用。结果 与正常人纤维细胞相比,miR-219-5p的表达水平显著降低,而SOX5的表达水平显著增加,差异有统计学意义(P <0.05);miR-219-5p与SOX5的表达水平具有负相关(P <0.05);在SCC4及SCC9细胞中,miR-219-5p通过结合3’非编码区(3’-UTR)靶向调控SOX5的表达。结论 miR-219-5p可能通过对SOX5的靶向调控参与了口腔鳞状细胞癌的发生发展,为协助临床预后评估和/或筛选治疗的新候选药物提供了科学依据。Objectives To explore whether the expressions of miR-219-5p and/or SRY-related highmobility-group box 5(SOX5) in oral squamous cell carcinoma cells are differ from normal cells. We also aim to confirm the transcription regulation in SOX5 by miR-219-5p. Methods Human oral squamous cell lines SCC4 and SCC9 were employed. The expression levels of miR-219-5p and SOX5 were detected in cancer cells(indicated by RT-qPCR and Western blot). Genetic analysis and luciferase assay were employed to verify whether miR-219-5p targets SOX5 by binding to the specific region of 3’-UTR. Results Our data showed that high miR-219-5p and low SOX5 levels were detected in vitro SCC4 and SCC9 cells(P < 0.05). miR-219-5p was negatively correlated with SOX5 expression. Genetic analysis and luciferase assay revealed that miR-291-5p regulated SOX5 expression by targeting the 3’untranslated region(3’-UTR) of the SOX5 mRNA. Conclusions This study demonstrates that miR-219-5p is involved in the development and prognosis of oral cancer by targeting the expression of SOX5,and can be a novel candidate for clinical prognosis and/or therapy.

关 键 词：口腔癌细胞 miR-219-5p SOX5 靶向调控 

分 类 号：R739.8[医药卫生—肿瘤]