A 314-bp SINE insertion in the ZNF2 promoter region may act as a repressor related to regulation of fat deposition in pigs  

作　　者：GU Hao DU Zhan-yu Eduard MURANI Enrico D'ALESSANDRO CHEN Cai WANG Xiao-yan MAO Jiu-de Klaus WIMMERS SONG Cheng-yi 

机构地区：[1]College of Animal Science&Technology,Yangzhou University,Yangzhou 225009,P.R.China [2]Leibniz Institute for Farm Animal Biology(FBN),Dummerstorf 18196,Germany [3]Department of Veterinary Science,Unit of Animal Production,University of Messina,Messina 98168,Italy [4]Bond Life Science Center,University of Missouri,Columbia,MO,65211,USA

出　　处：《Journal of Integrative Agriculture》2023年第2期526-536,共11页农业科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(32002146 and 31872977);the China Postdoctoral Science Foundation(2020M671630);the Jiangsu Postdoctoral Science Foundation of China(2021K221B)to Chen Cai;the Jiangsu Agriculture Science and Technology Innovation Fund,China[CX(19)2016];the Priority Academic Program Development of Jiangsu Higher Education Institutions;the High-end Talent Support Program of Yangzhou University,China to Song Chengyi。

摘　　要：Retrotransposons,a type of DNA fragment that can mobilize itself on genome,can generate genetic variations and develop for molecular markers based on the insertion polymorphism.Zinc finger proteins(ZNFs)are among the most abundant proteins in eukaryotic animals,and their functions are extraordinarily diverse and particularly important in gene regulation.In the current study,bioinformatic prediction was performed to screen for retrotransposon insertion polymorphisms(RIPs)in six ZNF genes(ZNF2,ZNF3,ZNF7,ZNF8,ZNF10 and ZNF12).Six RIPs in these ZNFs,including one short interspersed nuclear element(SINE)RIP in intron 1 and one long interspersed nuclear element 1(L1)RIP in intron 3 of ZNF2,one SINE RIP in 5′flanking region and one SINE RIP in intron 2 of ZNF3,one SINE RIP in 3′UTR of ZNF7 and one L1 RIP in intron 2 of ZNF12,were discovered and their presence was confirmed by PCR.The impact of the SINE RIP in the first intron of ZNF2,which is close to the core promoter of ZNF2,on the gene activity was investigated by dual-luciferase assay in three cell lines.Our results showed that the SINE insertion in the intron 1 of ZNF2 repressed the core promoter activity extremely significantly(P<0.01)in cervical cancer cells and porcine primary embryonic fibroblasts(HeLa and PEF),thus SINE may act as a repressor.This SINE RIP also significantly(P<0.05)affected the corrected back fat thickness in Yorkshire pigs.The corrected back fat thickness of individuals with SINE insertion in the first intron of ZNF2 was significantly(P<0.05)higher than that of individuals without SINE insertion.In summary,our data suggested that RIPs play important roles in the genetic variations of these ZNF genes and SINE RIP in the intron 1 of ZNF2 may provide a useful molecular marker for the screening of fat deposition in the pig breeding.

关 键 词：RETROTRANSPOSON ZNF2 POLYMORPHISM REPRESSOR fat deposition 

分 类 号：S858.28[农业科学—临床兽医学]